%A Guigen Ma, Yongjie Kuang, Zhenwan Lu, Xueqi Li, Ziyan Xu, Bin Ren, Xueping Zhou and Huanbin Zhou
%T CRISPR/Sc++-mediated genome editing in rice
%0 Journal Article
%D 2021
%J J Integr Plant Biol
%R 10.1111/jipb.13166
%P 1606-1610
%V 63
%N 9
%U {https://www.jipb.net/CN/abstract/article_81788.shtml}
%8 2021-09-01
%X Streptococcus canis Cas9 (ScCas9) is an RNA-guided endonuclease with NNG protospacer adjacent motif (PAM) specificity whose genome-editing activity in rice is locus-dependent. Here we investigated the performance of a ScCas9 variant named Sc++ at different NNG PAM sites in the rice genome; Sc++ harbors a T1227K mutation and the substitution of a positively charged loop (residues 367–376). Sc++ nuclease achieved broader genome editing compared to the original ScCas9, and its nickase improved targeted base editing in transgenic rice plants. Using the high-efficiency adenine base editor rBE73b, we generated many new OsGS1 alleles suitable for screening of rice germplasm for potential herbicide resistance in the future. The CRISPR/Sc++ system expands the genome-editing toolkit for rice.