Please wait a minute...
[an error occurred while processing this directive]
J Integr Plant Biol, 47 (3): -, Research Article
Antiporter Gene from Hordum brevisubulatum (Trin.) Link and Its Overexpression in Transgenic Tobaccos
Shi-You LÜ, Yu-Xiang JING, Shi-Hua SHEN, Hua-Yan ZHAO, Lan-Qing MA, Xiang-Juan ZHOU, Qing REN,Yan-Fang LI
Abstract
A vacuolar Na+/H+ antiporter cDNA gene was successfully isolated from Hordeum brevisubulatum (Trin.) Link using the rapid amplification of cDNA ends (RACE) method. The gene was named HbNHX1 and was found to consist of 1 916 bp encoding a predicted polypeptide of 540 amino acids with a conserved amiloride-binding domain. Phylogenetic tree analysis of the Na+/H+ antiporters showed that the HbNHX1 gene shares 55.3%–74.8% similarity with the vacuolar-type Na+/H+ antiporters. Transgenic tobaccos that contain the HbNHX1 gene, integrated by forward insertion into the tobacco genome, were obtained via Agrobacterium tumerfaciens and characterized for the determination of the concentration of Na+ and K+ ions, as well as proline, in the presence of 300 mmol/L NaCl. The T1 transgenic plants showed more tolerance to salt and drought than did wild-type plants. Our data suggest that overexpression of the HbNHX1 gene could improve the tolerance of transgenic tobaccos to salt and drought through the function of the vacuolar Na+/H+ antiporter.
---------------------------------------------------
Published: 02 March 2005
© 2005 Institute of Botany, Chinese Academy of Sciences
This Article
   Full Text
   Full Text (PDF)
   Supporting information
Services
   E-mail Link to this Article
   Export Citation for this Article
Citing Articles
   Cited By
Google Scholar
   Articles by Shi-You LÜ Yu-Xiang JING
   Articles by Shi-Hua SHEN
   Articles by Hua-Yan ZHAO
   Articles by Lan-Qing MA
   Articles by Xiang-Juan ZHOU
   Articles by Qing REN
   Articles by Yan-Fang LI
PubMed
   Articles by Shi-You LÜ Yu-Xiang JING
   Articles by Shi-Hua SHEN
   Articles by Hua-Yan ZHAO
   Articles by Lan-Qing MA
   Articles by Xiang-Juan ZHOU
   Articles by Qing REN
   Articles by Yan-Fang LI
[an error occurred while processing this directive]