Ectopic expression of fungal EcGDH improves nitrogen assimilation and grain yield in rice

doi:10.1111/jipb.12519

J Integr Plant Biol ›› 2018, Vol. 60 ›› Issue (2): 85-88.DOI: 10.1111/jipb.12519

Ectopic expression of fungal EcGDH improves nitrogen assimilation and grain yield in rice

Dongying Tang^1,2†, Yuchong Peng^2†, Jianzhong Lin^2†, Changqing Du², Yuanzhu Yang³, Dan Wang², Cong Liu², Lu Yan², Xiaoying Zhao², Xia Li¹, Liangbi Chen^1* and Xuanming Liu^2*

收稿日期:2016-12-28 接受日期:2017-01-05 出版日期:2018-02-13 发布日期:2017-01-06

Ectopic expression of fungal EcGDH improves nitrogen assimilation and grain yield in rice

Dongying Tang^1,2†, Yuchong Peng^2†, Jianzhong Lin^2†, Changqing Du², Yuanzhu Yang³, Dan Wang², Cong Liu², Lu Yan², Xiaoying Zhao², Xia Li¹, Liangbi Chen^1* and Xuanming Liu^2*

¹College of Life Sciences, Hunan Normal University, Changsha, China
²Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha, China
³Academy of Seed Industry of Hunan Yahua, Changsha, China

Received:2016-12-28 Accepted:2017-01-05 Online:2018-02-13 Published:2017-01-06
About author:^†These authors contributed equally to this work.
^**Correspondences: E-mail: Liangbi Chen (chenliangbi@126.com), Xuanming Liu (xmL05@hnu.edu.cn)

摘要/Abstract

摘要： Owing to the higher affinity for ammonium, NADP(H)-dependent glutamate dehydrogenase (EcGDH) from Eurotium cheralieri can more efficiently assimilate nitrogen than OsGDH from rice. Ectopic expression of EcGDH significantly enhanced the nitrogen assimilation efficiency, and especially improved grain yield in rice at low nitrogen fertility.

Abstract:

NADP(H)-dependent glutamate dehydrogenases (GDH) in lower organisms have stronger ammonium affinity than those in higher plants. Here we report that transgenic rice overexpressing the EcGDH from Eurotium cheralieri exhibited significantly enhanced aminating activities. Hydroponic and field tests showed that nitrogen assimilation efficiency and grain yields were markedly increased in these transgenic plants, especially at the low nitrogen conditions. These results suggest that EcGDH may have potential to be used to improve nitrogen assimilation and grain yield in rice.

Dongying Tang, Yuchong Peng, Jianzhong Lin, Changqing Du, Yuanzhu Yang, Dan Wang, Cong Liu, Lu Yan, Xiaoying Zhao, Xia Li, Liangbi Chen, and Xuanming Liu. Ectopic expression of fungal EcGDH improves nitrogen assimilation and grain yield in rice[J]. J Integr Plant Biol, 2018, 60(2): 85-88.

Ectopic expression of fungal EcGDH improves nitrogen assimilation and grain yield in rice

Ectopic expression of fungal EcGDH improves nitrogen assimilation and grain yield in rice

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