J Integr Plant Biol ›› 2025, Vol. 67 ›› Issue (5): 1214-1216.DOI: 10.1111/jipb.13842

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  • 收稿日期:2024-09-21 接受日期:2024-12-17 出版日期:2025-05-01 发布日期:2025-05-12

Generation of novel bpm6 and dmr6 mutants with broad-spectrum resistance using a modified CRISPR/Cas9 system in Brassica oleracea

Yulun Zhang1,2†, Jinhui Liu1†, Yingjie Li1, Hongxue Ma1, Jialei Ji1, Yong Wang1, Mu Zhuang1, Limei Yang1, Zhiyuan Fang1, Jun Li3, Chao Zhang3, Liwang Liu4, Marina Lebedeva5, Vasiliy Taranov5, Yangyong Zhang1* and Honghao Lv1,2*   

  1. 1. State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081 China
    2. College of Plant Science and Technology, Beijing University of Agriculture, Beijing 100096 China
    3. College of Life Sciences, Hebei Agricultural University, Baoding 071001 China
    4. College of Horticulture, Nanjing Agricultural University, Nanjing 210095 China
    5. All‐Russia Research Institute of Agricultural Biotechnology, Russian Academy of Sciences, Moscow 125319 Russia

    †These authors contributed equally to this work
    *Correspondences: Yangyong Zhang (zhangyangyong@caas.cn); Honghao Lv (lvhonghao@caas.cn; Dr. Lv is fully responsible for the distribution of all materials associated with this article)
  • Received:2024-09-21 Accepted:2024-12-17 Online:2025-05-01 Published:2025-05-12
  • Supported by:
    This work was supported by grants from the National Key R&D Program of China (2023YFE0111400), the Key Technology R&D Program of Jiangsu Province (BE2023366), S&T Program of Hebei Province (22322912D), the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS‐ASTIP‐IVFCAAS), the China Agriculture Research System of MOF and MARA (CARS‐23), and Wulanchabu City's Challenge and Recruitment Project (2022JB006). The work was also partly supported by the Russian Ministry of Science and Higher Education (No.075‐15‐2023‐582).
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