J Integr Plant Biol ›› 2025, Vol. 67 ›› Issue (5): 1274-1289.DOI: 10.1111/jipb.13845

• • 上一篇    下一篇

  

  • 收稿日期:2024-06-14 接受日期:2024-12-29 出版日期:2025-05-01 发布日期:2025-05-12

Arabidopsis CIRP1 E3 ligase modulates drought and oxidative stress tolerance and reactive oxygen species homeostasis by directly degrading catalases

Heng Yang1,2†, Yi Zhang1†, Shanwu Lyu1, Yaping Mao1,3, Fangqin Yu1,2, Sai Liu1,2, Yujie Fang1,3 and Shulin Deng1,2,3*   

  1. 1. Key Laboratory of National Forestry and Grassland Administration on Plant Conservation and Utilization in Southern China & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
    2. University of Chinese Academy of Sciences, Beijing 100049, China
    3. National Engineering Research Center of Navel Orange, College of Life Sciences, Gannan Normal University, Ganzhou 341000, China

    These authors contributed equally to this work.
    *Correspondence: Shulin Deng (sldeng@scbg.ac.cn)
  • Received:2024-06-14 Accepted:2024-12-29 Online:2025-05-01 Published:2025-05-12
  • Supported by:
    This work was supported by grants from the National Natural Science Foundation of China (32170304 and 32070340) and the Natural Science Foundation of Guangdong Province (2022A1515012572).

Abstract: Reactive oxygen species (ROS) plays critical roles in modulating plant growth and stress response and its homeostasis is fine tuned using multiple peroxidases. H2O2, a major kind of ROS, is removed rapidly and directly using three catalases, CAT1, CAT2, and CAT3, in Arabidopsis. Although the activity regulations of catalases have been well studied, their degradation pathway is less clear. Here, we report that CAT2 and CAT3 protein abundance was partially controlled using the 26S proteasome. To further identify candidate proteins that modulate the stability of CAT2, we performed yeast-two-hybrid screening and recovered several clones encoding a protein with RING and vWA domains, CIRP1 (CAT2 Interacting RING Protein 1). Drought and oxidative stress downregulated CIRP1 transcripts. CIRP1 harbored E3 ubiquitination activity and accelerated the degradation of CAT2 and CAT3 by direct interaction and ubiquitination. The cirp1 mutants exhibited stronger drought and oxidative stress tolerance, which was opposite to the cat2 and cat3 mutants. Genetic analysis revealed that CIRP1 acts upstream of CAT2 and CAT3 to negatively regulate drought and oxidative stress tolerance. The increased drought and oxidative stress tolerance of the cirp1 mutants was due to enhanced catalase (CAT) activities and alleviated ROS levels. Our data revealed that the CIRP1–CAT2/CAT3 module plays a vital role in alleviating ROS levels and balancing growth and stress responses in Arabidopsis.

Key words: CAT2, CIRP1, drought tolerance, oxidative tolerance, reactive oxygen species, ubiquitination

[an error occurred while processing this directive]