J Integr Plant Biol ›› 2025, Vol. 67 ›› Issue (7): 1965-1981.DOI: 10.1111/jipb.13921

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  • 收稿日期:2025-04-13 接受日期:2025-04-05 出版日期:2025-07-01 发布日期:2025-07-07

OsPRK1/2/3-mediated reactive oxygen species signaling is required for pollen tube germination in rice

Eui‐Jung Kim1†, Ye‐jin Son2†, Ji‐Hyun Kim2, Woo‐Jong Hong3, Su Kyoung Lee1, Sun Tae Kim4, Wanqi Liang5, Sunok Moon1, Yu‐Jin Kim2* and Ki‐Hong Jung1*   

  1. 1. Graduate School of Green‐Bio Science & Crop Biotech Institute, Kyung Hee University, Yongin 17104, Korea
    2. Department of Life Science and Environmental Biochemistry, and Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Korea
    3. Department of Smart Farm Science, Kyung Hee University, Yongin 17104, Korea
    4. Department of Plant Bioscience, Pusan National University, Miryang 50463, Korea
    5. Joint International Research Laboratory of Metabolic & Developmental Sciences, State Key Laboratory of Hybrid Rice, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China

    †These authors contributed equally to this manuscript.
    *Correspondences: Yu‐Jin Kim (yjkim2020@pusan.ac.kr); Ki‐Hong Jung (khjung2010@khu.ac.kr, Dr. Jung is fully responsible for the distribution of all materials associated with this article)
  • Received:2025-04-13 Accepted:2025-04-05 Online:2025-07-01 Published:2025-07-07
  • Supported by:
    This research was supported by grants from the National Research Foundation (NRF), Ministry of Education, Science and Technology (RS‐2023‐00275589 and RS‐2023‐00217064 to Y.‐J.K., RS‐2025‐00555009 to E.‐J.K., and RS‐2021‐NR060084 to K.‐H.J.), and the New Breeding Technologies Development Program (RS‐2024‐00322278 to S.M.) from the Rural Development Administration (RDA), Republic of Korea.

Abstract: Pollen hydration, germination, and tube growth are vital processes for the successful fertilization of flowering plants. These processes involve complex signaling pathways. Reactive oxygen species (ROS) generated in apoplast involves signaling for the cell wall expansion during tube growth, however molecular regulators are less known. We identified pollen-specific receptor-like kinase (OsPRK) family genes from rice (Oryza sativa), which have conserved leucine-rich repeat (LRR) and kinase domains. To understand the function of these genes, we produced single and triple mutations for OsPRK1, OsPRK2, and OsPRK3 using the clustered regularly interspaced palindromic repeats (CRISPR/Cas9) system. Among these mutants, triple knockout (KO) lines (osprk1/2/3) exhibited the male-sterile phenotype with normal vegetative growth and floret formation. Through cytological analysis, we confirmed that the reduced seed fertility was due to defects in pollen hydration and germination with low ROS accumulation. This defect of pollen germination was partially recovered by treatment with exogenous H2O2. We also confirmed that OsPRKs could interact with the LRR extension protein. Our results suggest that rice PRKs redundantly play a role in ROS signaling for pollen hydration and germination, and fertility can be controlled by exogenous application.

Key words: CRISPR/Cas9, Oryza sativa, pollen tube germination, pollen‐specific receptor‐like kinase (PRK), reactive oxygen species

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