J Integr Plant Biol ›› 2026, Vol. 68 ›› Issue (1): 191-202.DOI: 10.1111/jipb.70058

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  • 收稿日期:2024-07-21 接受日期:2025-09-19 出版日期:2026-01-01 发布日期:2026-01-12

CRY2 interacts with PRR5 in a blue light-dependent manner to mediate blue light-regulated circadian period

Chen Shi1,2,3†, Xisen Yuan1,2,3†, Libang Ma2,3, Tangtao Wen1, Xiaoyu Jia1,4, Xu Li1,2 and Hongtao Liu1,2,5*   

  1. 1. Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China
    2. National Key Laboratory of Plant Molecular Genetics (NKLPMG), CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology (SIPPE), the Chinese Academy of Sciences, Shanghai 200032, China
    3. University of Chinese Academy of Sciences, Shanghai 200032, China
    4. College of Life Sciences, Henan University, Kaifeng 475001, China
    5. Synthetic Biology Research Center, Shenzhen University, Shenzhen 518060, China

    †These authors contributed equally to this work.
    *Correspondence: Hongtao Liu (htliu@szu.edu.cn, Dr. Liu is fully responsible for the distribution of all materials associated with this article)
  • Received:2024-07-21 Accepted:2025-09-19 Online:2026-01-01 Published:2026-01-12
  • Supported by:
    This work was supported in part by the National Key R&D Program of China (2024YFA1306700), the National Natural Science Foundation of China (32330006, 32150007, 31825004, 32200229, 32170247), the Research Team Cultivation Program of Shenzhen University (2023DFT005 to H.L.).

Abstract: Cryptochromes (CRYs) are photolyase-like blue-light receptors originally identified in Arabidopsis thaliana (Arabidopsis) and have since been identified across diverse evolutionary lineages. Cryptochromes not only transduce blue-light cues to the circadian clock but also maintain the temperature compensation of circadian clock. However, the precise mechanism by which CRYs integrate blue light signals into the circadian clock in Arabidopsis is still under investigation. This study revealed that, when blue light was filtered out from white light, the circadian period length in Col-0 was extended, but not in the cry1 cry2 double mutant. This indicates that both blue light and CRYs are crucial for regulating the circadian rhythm. Furthermore, we discovered that CRY2 interacted with PSEUDORESPONSE REGULATOR 5 (PRR5), a key component of the circadian clock under blue light, which suppressed PRR5's transcriptional inhibition ability on CCA1 and LHY. These findings illuminate the pathway through which blue light influences the circadian clock via the CRY2–PRR5 module.

Key words: blue light, CCA1, circadian clock, CRY2, LHY, PRR5

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