Interspecies rice versus Arabidopsis thaliana protein–protein interactome profiling by touch-down overlapping PCR coupled with HiFi long-read sequencing

doi:10.1111/jipb.70107

J Integr Plant Biol ›› 2026, Vol. 68 ›› Issue (2): 294-296.DOI: 10.1111/jipb.70107

收稿日期:2025-09-16 接受日期:2025-11-13 出版日期:2026-02-02 发布日期:2026-02-03

Interspecies rice versus Arabidopsis thaliana protein–protein interactome profiling by touch-down overlapping PCR coupled with HiFi long-read sequencing

Jie Huang^1†, Yu Cheng^1†, Jing Ruan^1,2†, Xixi Liu¹, Dandan Xia³, Yiting Chen¹, Delong Fan¹, Jiezheng Ying¹, Yifeng Wang¹, Xiaohong Tong¹, Zhiyong Li¹, Dawei Xue², Jianwei Zhang³, Jian Zhang^1* and Yuxuan Hou^1*

1. State Key Lab of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, China

2. College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China

3. National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China

^†These authors contributed equally.

*Correspondences: Jian Zhang (zhangjian@caas.cn), Yuxuan Hou (houyuxuan@caas.cn, Dr. Houis is fully responsible for the distribution of the materialsassociated with this article)

Received:2025-09-16 Accepted:2025-11-13 Online:2026-02-02 Published:2026-02-03
Supported by:
This work was supported by Biological Breeding‐National Science and Technology Major Project (Grant No. 2023ZD040662), National Key R&D Program of China (Grant No. 2024YFF1000600), Zhejiang Provincial Natural Science Foundation (Grant No. LQN25C130011, LY23C130004), Natural Science Foundation of China (Grant No. 32472115, U22A20456 and W2412006), Major Program of Natural Science Foundation of Zhejiang (Grant No. LD24C130001), and ASTIP program of CAAS.

摘要/Abstract

. [J]. J Integr Plant Biol, 2026, 68(2): 294-296.

Jie Huang, Yu Cheng, Jing Ruan, Xixi Liu, Dandan Xia, Yiting Chen, Delong Fan, Jiezheng Ying, Yifeng Wang, Xiaohong Tong, Zhiyong Li, Dawei Xue, Jianwei Zhang, Jian Zhang, Yuxuan Hou. Interspecies rice versus Arabidopsis thaliana protein–protein interactome profiling by touch-down overlapping PCR coupled with HiFi long-read sequencing[J]. J Integr Plant Biol, 2026, 68(2): 294-296.

Interspecies rice versus Arabidopsis thaliana protein–protein interactome profiling by touch-down overlapping PCR coupled with HiFi long-read sequencing

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