Abstract: Unpollinated young ovaries of lily (Lilium davidii var. willmottiae (Wilson) Roffill) were inoculated on modified MS medium and N6 medium. Ovary cultures were incubated at 25–28℃, and illuminated with a fluorescent light of about 800–1200 Lux. Cultured ovaries gradually became thicker and longer after 10 days. The calli (about 6–12 mm in size) were produced after 40 days. The calli were then transferred to the differentiation medium. After 50 days, regeneration plantlets were formed. Embryoids were directly produced from some ovaries, which then developed into plantlets. Observation of chromosome number of regeneration plants shows: 65.71% regeneration plants are haploid plants, 34.29% are diploid. Embryological observation of ovary culture shows that haploid plants are from megaspore tetrad, while diploid plants are probably from nucellus cell.