Using Subtracted AFLP to Efficiently Mark an Alien Chromosome Fragment in Wheat Background

J Integr Plant Biol ›› 2003, Vol. 45 ›› Issue (4): 379-383.

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Using Subtracted AFLP to Efficiently Mark an Alien Chromosome Fragment in Wheat Background

CHAI Jian-Fang, WU Zhi-Ming, ZHAO He, André LAROCHE, WANG Hai-Bo

出版日期:2003-04-15 发布日期:2003-04-15

Using Subtracted AFLP to Efficiently Mark an Alien Chromosome Fragment in Wheat Background

CHAI Jian-Fang, WU Zhi-Ming, ZHAO He, André LAROCHE, WANG Hai-Bo

Online:2003-04-15 Published:2003-04-15

摘要/Abstract

Abstract:

利用减法AFLP有效标记小麦中的外源染色体片段
柴建芳吴志明赵和王海波

（1. 河北省农林科学院农业物理生理生化研究所，石家庄050051；
2. Agriculture and Agri-F00d Canada, Lethbridge Research Center,Lethbridge, AB T1j4B1,Canada）

摘要：为解决AFLP中样品间共同片段对多态性片段识别的干扰,作者建立了一种"减法AFLP"标记技术,并运用这种技术成功对小麦中的外源染色体片段进行了标记.运用该技术,样品间的共同扩增片段显著减少,在非变性聚丙稀酰胺凝胶上带型差异十分明显,多态性片段很容易分辨.其真实性通过将其中两条成功转换成外源染色体片段特异的SCAR标记得到了验证.减法AFLP标记技术的建立为标记作物中的外源染色体片段提供了一种有效手段.

关键词：减法AFLP；减法杂交；AFLP；外源染色体片段；小麦

通讯作者。Tel: 0311-7652006; E-mail: pgec @ heinfo.net。

CHAI Jian-Fang, WU Zhi-Ming, ZHAO He, André LAROCHE, WANG Hai-Bo. Using Subtracted AFLP to Efficiently Mark an Alien Chromosome Fragment in Wheat Background[J]. J Integr Plant Biol, 2003, 45(4): 379-383.

Using Subtracted AFLP to Efficiently Mark an Alien Chromosome Fragment in Wheat Background

Using Subtracted AFLP to Efficiently Mark an Alien Chromosome Fragment in Wheat Background

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