]*>","")" /> A Comparative Study on Detection of the Expression of Alternative Oxidase in Tobacco Callus with the Monoclonal Antibody Against Alternative Oxidase and Antibody Against Synthetic Polypeptide Antibody

J Integr Plant Biol ›› 2002, Vol. 44 ›› Issue (10): 1255-1257.

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A Comparative Study on Detection of the Expression of Alternative Oxidase in Tobacco Callus with the Monoclonal Antibody Against Alternative Oxidase and Antibody Against Synthetic Polypeptide Antibody

YAN Ying Cai, LIN Hong Hui*, LIANG Hou Guo, DU Lin Fang   

  • 出版日期:2002-10-21 发布日期:2002-10-21

A Comparative Study on Detection of the Expression of Alternative Oxidase in Tobacco Callus with the Monoclonal Antibody Against Alternative Oxidase and Antibody Against Synthetic Polypeptide Antibody

YAN Ying Cai, LIN Hong Hui*, LIANG Hou Guo, DU Lin Fang   

  • Online:2002-10-21 Published:2002-10-21

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Abstract:

单克隆抗体和抗合成多肽抗体检测烟草愈伤组织中交替氧化酶表达的比较
晏婴才 林宏辉*  梁厚果 杜林方
（四川大学生命科学学院，成都610064I）


摘要： 从经过不同温度处理的烟草（Nicotiana rustica L.）愈伤组织中提取并纯化线粒体蛋白，分别与交替氧化酶的单克隆抗体和抗合成多肽抗体进行免疫杂交。结果表明：交替氧化酶的含量随温度的下降而显著上升；单克隆抗体的特异性较高于抗合成多肽抗体，但后者与交替氧化酶同样有良好的亲和性。因此，用合成多肽方法制备的抗体可以用于交替氧化酶的研究中。
关键词： 交替氧化酶；单克隆抗体；抗合成多肽抗体；免疫杂交；烟草愈伤组织

Key words: alternative oxidase, monoclonal antibody, anti synthetic polypeptide antibody, immunoblot, tobacco callus

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