Abstract:

To isolate genes related to resistance to Erysiphe graminis DC. ex Merat f.sp. tritici Em. Marchal in wheat (Triticum aestivum L.), differential display analysis was conducted for mRNA extracted from the seedlings of the wheat Haynaldia villosa 6VS/6A L translocation line 92R137 that contains the powdery mildew resistance gene Pm21. A full length cDNA named TaPK1 was isolated. BLAST analysis revealed that it was significantly homologous to Glycine max (L.) Merr. protein kinase (GmPK6) cDNA. TaPK1 encodes a 416 amino acid long polypeptide, which belongs to serine/threonine protein kinase family, also has tyrosine kinase specificity. TaPK1 is a novel protein kinase from wheat. 

一个小麦丝氨酸-苏氨酸蛋白激酶基因的克隆和分析

牛吉山  于玲  马正强  陈佩度^*  刘大钧

(南京农业大学农业部作物细胞遗传重点实验室,南京,210095)

用mRNA差异显示技术在含有抗白粉病基因Pm21的小麦(Tri ticum aestivum L.) -簇毛麦(Haynaldia villosa) 6VS /6AL易位系92R137中分离与抗白粉病相关的基因,获得一个命名为TaPK1的全长cDNA克隆.序列分析表明,它与大豆(Glycine max (L.) Merr.)蛋白激酶基因GmPK6高度同源.经推测,TaPK1 编码416个氨基酸的多肽,属丝氨酸-苏氨酸蛋白激酶家族,并具酪氨酸激酶特性.TaPK1是从小麦中分离的新基因.

关键词 小麦 白粉病 蛋白激酶 

*通讯作者 Email: pdchen@mail.njau.edu.cn

Key words: wheat, powdery mildew, protein kinase