Abstract:

By means of paraformaldehyde fixation, Triton X-100 extraction and TRITC-phalloidin staining, the presence and distribution patterns of F-actin in the outer epidermal cells of the garlic (Allium sativum L.) sheath were studied with fluorescence probe technique and confocal laser scanning microscopy. There were a lot of actin filaments (AFs) impenetrate the cell wall, but the AFs with red fluorescence were absent when the cells were treated with cytochalasin D before fixation; the same result was obtained when the cells were treated with unlabeled phalloidin. These results indicate the presence of F-actin in the intercellular channels and that it is related to the plasmodesmata and intercellular trafficking of macromolecules.

蒜瓣鞘表皮组织中肌动蛋白纤丝跨胞分布的共焦荧光显微镜观察
王冬梅1,2 王学臣2* 张伟成3

（1. 河北农业大学农学院，保定071001；2.  中国农业大学生物学院，北京100094；#$ 中国科学院上海植物生理研究所，上海200032）
关键词： 胞间连丝；F-肌动蛋白；异硫氰四甲基罗丹明*鬼笔环肽；细胞松弛素-D；激光共聚焦扫描显微镜检术；蒜

Key words: plasmodesmata, F-actin, TRITC-phalloidin, cytochalasin D, confocal laser scanning microscopy (CLSM), Allium sativum