J Integr Plant Biol ›› 2017, Vol. 59 ›› Issue (9): 594-599.DOI: 10.1111/jipb.12556

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Arabidopsis adaptor protein 1G is critical for pollen development

Chong Feng†, Jia-Gang Wang†, Hai-Hong Liu, Sha Li* and Yan Zhang*   

  • 收稿日期:2017-04-12 接受日期:2017-05-22 出版日期:2017-05-24 发布日期:2017-05-24

Arabidopsis adaptor protein 1G is critical for pollen development

Chong Feng†, Jia-Gang Wang†, Hai-Hong Liu, Sha Li* and Yan Zhang*   

  1. State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China
  • Received:2017-04-12 Accepted:2017-05-22 Online:2017-05-24 Published:2017-05-24
  • About author:†These authors contributed equally to this work.
    **Correspondences: E-mail: Sha Li (shali@sdau.edu.cn), Yan Zhang (yzhang@sdau.edu.cn, Dr. Zhang is fully responsible for distributions of all materials associated with this article)

摘要： AP1G, adaptor protein 1, is essential for protein sorting at the trans-Golgi network/early endosomes. This study demonstrates that functional loss of the γ subunit of AP1G resulted in male gametophytic lethality by affecting vacuolar remodeling and acidification during pollen development.

Abstract:

Pollen development is a pre-requisite for sexual reproduction of angiosperms, during which various cellular activities are involved. Pollen development accompanies dynamic remodeling of vacuoles through fission and fusion, disruption of which often compromises pollen viability. We previously reported that the Y subunit of adaptor protein 1 (AP1G) mediates synergid degeneration during pollen tube reception. Here, we demonstrate that AP1G is essential for pollen development. AP1G loss-of-function resulted in male gametophytic lethality due to defective pollen development. By ultrastructural analysis and fluorescence labeling, we demonstrate that AP1G loss-of-function compromised dynamic vacuolar remodeling during pollen development and impaired vacuolar acidification of pollen. Results presented here support a key role of vacuoles in gametophytic pollen development.

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