J Integr Plant Biol ›› 2017, Vol. 59 ›› Issue (2): 102-117.DOI: 10.1111/jipb.12514

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A novel tomato SUMO E3 ligase, SlSIZ1, confers drought tolerance in transgenic tobacco

Song Zhang, Kunyang Zhuang, Shiju Wang, Jinlian Lv, Na'na Ma* and Qingwei Meng*   

  • 收稿日期:2016-11-25 接受日期:2016-12-19 出版日期:2016-12-20 发布日期:2016-12-20

A novel tomato SUMO E3 ligase, SlSIZ1, confers drought tolerance in transgenic tobacco

Song Zhang, Kunyang Zhuang, Shiju Wang, Jinlian Lv, Na'na Ma* and Qingwei Meng*   

  1. State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, China
  • Received:2016-11-25 Accepted:2016-12-19 Online:2016-12-20 Published:2016-12-20
  • About author:**Correspondences:
    E-mail: Na'na Ma (nnma@sdau.edu.cn), Qingwei Meng (qwmeng@sdau.edu.cn)(Dr. Meng is fully responsible for the distribution of all materials associated with this article)

被引次数

9

摘要： The tomato gene SlSIZ1 was isolated and the SlSIZ1 protein was identified as a functional homolog of Arabidopsis SIZ1 with SUMO E3 ligase activity. Overexpression of SlSIZ1 can enhance the drought tolerance of transgenic tobacco by increasing the levels of SUMO conjugates and osmotic materials, and decreasing membrane damage from ROS.

Abstract:

SUMOylation is an important post-translational modification process that regulates different cellular functions in eukaryotes. SIZ/PIAS-type SAP and Miz1 (SIZ1) proteins exhibit SUMO E3 ligase activity, which modulates SUMOylation. However, SIZ1 in tomato has been rarely investigated. In this study, a tomato SIZ1 gene (SlSIZ1) was isolated and its molecular characteristics and role in tolerance to drought stress are described. SlSIZ1 was up-regulated by cold, sodium chloride (NaCl), polyethylene glycol (PEG), hydrogen peroxide (H2O2) and abscisic acid (ABA), and the corresponding proteins were localized in the nucleus. The expression of SlSIZ1 in Arabidopsis thaliana (Arabidopsis) siz1-2 mutants partially complemented the phenotypes of dwarf, cold sensitivity and ABA hypersensitivity. SlSIZ1 also exhibited the activity of SUMO E3 ligase to promote the accumulation of SUMO conjugates. Under drought stress, the ectopic expression of SlSIZ1 in transgenic tobacco lines enhanced seed germination and reduced the accumulation of reactive oxygen species. SlSIZ1 overexpression conferred the plants with improved growth, high free proline content, minimal malondialdehyde accumulation and increased accumulation of SUMO conjugates. SlSIZ1 is a functional homolog of Arabidopsis SIZ1 with SUMO E3 ligase activity. Therefore, overexpression of SlSIZ1 enhanced the tolerance of transgenic tobacco to drought stress.

Key words: drought stress, SUMO E3 ligase SlSIZ1, SUMO conjugates, tomato, transgenic tobacco

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