J Integr Plant Biol ›› 2016, Vol. 58 ›› Issue (7): 618-622.DOI: 10.1111/jipb.12457

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Depletion of tyrosyl-DNA phosphodiesterase 1α (MtTdp1α) affects transposon expression in Medicago truncatula

Maria Elisa Sabatini1, Mattia Donà1†, Paola Leonetti2, Andrea Minio3, Massimo Delledonne3, Daniela Carbonera1, Massimo Confalonieri4, Giorgio Giraffa4 and Alma Balestrazzi1*   

  • 收稿日期:2015-04-20 接受日期:2015-12-18 出版日期:2015-12-23 发布日期:2015-12-23

Depletion of tyrosyl-DNA phosphodiesterase 1α (MtTdp1α) affects transposon expression in Medicago truncatula

Maria Elisa Sabatini1, Mattia Donà1†, Paola Leonetti2, Andrea Minio3, Massimo Delledonne3, Daniela Carbonera1, Massimo Confalonieri4, Giorgio Giraffa4 and Alma Balestrazzi1*   

  1. 1Department of Biology and Biotechnology ‘L. Spallanzani‘ 27100 Pavia, Italy
    2Institute for Sustainable Plant Protection, National Research Council (CNR), Bari, Italy
    3Department of Biotechnology, University of Verona, Verona, Italy
    4Fodder and Dairy Production Research Centre, Lodi, Italy
  • Received:2015-04-20 Accepted:2015-12-18 Online:2015-12-23 Published:2015-12-23
  • About author:†Present address: Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna Biocenter (VBC), Dr. Bohr Gasse 3, 1030 Vienna, Austria
    *Correspondence: E-mail: alma.balestrazzi@unipv.it

Abstract:

The role of plant tyrosyl-DNA phosphodiesterase 1α in genome stability is studied using a Medicago truncatula MtTdp1α-depleted line. Lack of MtTdp1α results in a 39% reduction of methylated cytosines as compared to control. RNA-Seq analyses revealed that 11 DNA transposons and 22 retrotransposons were differentially expressed in the Tdp1α-2a line. Among them all, DNA transposons (MuDR, hAT, DNA3-11_Mad) and seven retrotransposons (LTR (Long Terminal Repeat)/Gipsy, LTR/Copia, LTR and NonLTR/L1) were down-regulated, while the 15 retrotransposons were up-regulated. Results suggest that the occurrence of stress-responsive cis-elements as well as changes in the methylation pattern at the LTR promoters might be responsible for the enhanced retrotransposon transcription.

Key words: DNA methylation, Medicago truncatula, RNA-Seq, transposable elements, tyrosyl-DNA phosphodiesterase

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