J Integr Plant Biol ›› 2016, Vol. 58 ›› Issue (5): 466-474.DOI: 10.1111/jipb.12443

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Natural variation of H3K27me3 modification in two Arabidopsis accessions and their hybrid

Mei Yang1, Xuncheng Wang2, Hao Huang2, Diqiu Ren1, Yan'e Su3, Pan Zhu1, Danmeng Zhu1, Liumin Fan1, Liangbi Chen3, Guangming He1* and Xing Wang Deng1*   

  • 收稿日期:2015-09-16 接受日期:2015-10-20 出版日期:2015-10-24 发布日期:2015-10-24

Natural variation of H3K27me3 modification in two Arabidopsis accessions and their hybrid

Mei Yang1, Xuncheng Wang2, Hao Huang2, Diqiu Ren1, Yan'e Su3, Pan Zhu1, Danmeng Zhu1, Liumin Fan1, Liangbi Chen3, Guangming He1* and Xing Wang Deng1*   

  1. 1State Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center for Life Sciences, School of Advanced Agricultural Sciences and School of Life Sciences, Peking University, Beijing 100871, China
    2Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China
    3Department of Botany, College of Life Sciences, Hunan Normal University, Changsha 410081, China
  • Received:2015-09-16 Accepted:2015-10-20 Online:2015-10-24 Published:2015-10-24
  • About author:**Correspondence: E-mail: heguangming@pku.edu.cn; deng@pku.edu.cn (Dr. Deng is fully responsible for distributions of all materials associated with this article)

摘要： We performed genome-wide analyses of H3K27me3 modification and gene expression for two Arabidopsis accessions and their hybrid. H3K27me3 modification patterns were different in two accessions. Moreover, a negative correlation of H3K27me3 modification variation with gene expression variation was observed between these two accessions.

Abstract:

Histone modifications affect gene expression, but the mechanism and biological consequence of natural variation in histone modifications remain unclear. Here, we generated genome-wide integrated maps of H3K27me3 modification and transcriptome for Col, C24 and their F1 hybrid. A total of 1,828 genomic regions showing variation in H3K27me3 modification between Col and C24 were identified, most of which were associated with genic regions. Natural variation of H3K27me3 modification between parents could result in allelic bias of H3K27me3 in hybrids. Furthermore, we found that H3K27me3 variation between Col and C24 was negatively correlated with gene expression differences between two accessions, especially with those arising from the cis-effect. Importantly, mutation of CLF, an Arabidopsis methyltransferase for H3K27, altered gene expression patterns between the parents. Together, these data provide insights into natural variation of histone modifications and their association with gene expression differences between Arabidopsis ecotypes.

Key words: Allelic bias, Arabidopsis, gene expression, H3K27me3, natural variation

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