J Integr Plant Biol ›› 2016, Vol. 58 ›› Issue (5): 442-451.DOI: 10.1111/jipb.12379

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ANAC005 is a membrane-associated transcription factor and regulates vascular development in Arabidopsis

Jun Zhao1,2, Jiang-Shu Liu1,2, Fu-Ning Meng1,2, Zhen-Zhen Zhang3, Hao Long1,2, Wen-Hui Lin1†, Xiao-Min Luo1, Zhi-Yong Wang4 and Sheng-Wei Zhu1*   

  • 收稿日期:2015-05-06 接受日期:2015-07-08 出版日期:2015-07-14 发布日期:2015-07-14

ANAC005 is a membrane-associated transcription factor and regulates vascular development in Arabidopsis

Jun Zhao1,2, Jiang-Shu Liu1,2, Fu-Ning Meng1,2, Zhen-Zhen Zhang3, Hao Long1,2, Wen-Hui Lin1†, Xiao-Min Luo1, Zhi-Yong Wang4 and Sheng-Wei Zhu1*   

  1. 1Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China
    2University of Chinese Academy of Sciences, Beijing, China
    3Institute of Molecular Cell Biology, College of Life Science, Hebei Normal University, Shijiazhuang, China
    4Department of Plant Biology, Carnegie Institution for Science, Stanford, California, USA
  • Received:2015-05-06 Accepted:2015-07-08 Online:2015-07-14 Published:2015-07-14
  • About author:†Current address: School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
    *Correspondence: E-mail: zhusw@ibcas.ac.cn

摘要： ANAC005, a NAC family transcription factor, expresses mainly in vascular tissues. It localizes in cell membrane, whereas deletion of the last 20 amino acids caused its nuclear localization. Moreover, ANAC005 reduce xylem differentiation and decrease lignin synthesis.

Abstract:

Vascular tissues are very important for providing both mechanical strength and long-distance transport. The molecular mechanisms of regulation of vascular tissue development are still not fully understood. In this study we identified ANAC005 as a membrane-associated NAC family transcription factor that regulates vascular tissue development. Reporter gene assays showed that ANAC005 was expressed mainly in the vascular tissues. Increased expression of ANAC005 protein in transgenic Arabidopsis caused dwarf phenotype, reduced xylem differentiation, decreased lignin content, repression of a lignin biosynthetic gene and genes related to cambium and primary wall, but activation of genes related to the secondary wall. Expression of a dominant repressor fusion of ANAC005 had overall the opposite effects on vascular tissue differentiation and lignin synthetic gene expression. The ANAC005-GFP fusion protein was localized at the plasma membrane, whereas deletion of the last 20 amino acids, which are mostly basic, caused its nuclear localization. These results indicate that ANAC005 is a cell membrane-associated transcription factor that inhibits xylem tissue development in Arabidopsis.

Key words: ANAC005, Arabidopsis, NAC, vascular tissues development

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