J Integr Plant Biol ›› 2015, Vol. 57 ›› Issue (8): 708-721.DOI: 10.1111/jipb.12320

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Arabidopsis ROOT HAIR DEFECTIVE3 is involved in nitrogen starvation-induced anthocyanin accumulation

Jing Wang, Yan Wang, Ju Yang, Chunli Ma, Ying Zhang, Ting Ge, Zhi Qi and Yan Kang*   

  • 收稿日期:2014-10-07 接受日期:2014-12-05 出版日期:2014-12-10 发布日期:2014-12-10

Arabidopsis ROOT HAIR DEFECTIVE3 is involved in nitrogen starvation-induced anthocyanin accumulation

Jing Wang, Yan Wang, Ju Yang, Chunli Ma, Ying Zhang, Ting Ge, Zhi Qi and Yan Kang*   

  1. College of Life Sciences, Inner Mongolia University, Hohhot, China
  • Received:2014-10-07 Accepted:2014-12-05 Online:2014-12-10 Published:2014-12-10
  • About author:*Correspondence: E-mail: kangyan105@imu.edu.cn

摘要： We found a mutant showing anthocyanin overaccumulation phenotype under nitrogen starvation condition, was defective in root and root hair development. A combination of positional cloning and next generation mapping, we identified ROOT HAIR DEFECTIVE3 (RHD3) and found it achieves negative effect on the anthocyanin biosynthesis through participating in ethylene-regulating pathway.

Abstract:

Anthocyanin accumulation is a common phenomenon seen in plants under environmental stress. In this study, we identified a new allele of ROOT HAIR DEFECTIVE3 (RHD3) showing an anthocyanin overaccumulation phenotype under nitrogen starvation conditions. It is known that ethylene negatively regulates light- and sucrose-induced anthocyanin biosynthesis. We hypothesized that RHD3 achieves its negative effect on anthocyanin biosynthesis via an ethylene-regulating pathway. In support of this, similar to rhd3 mutants, the Arabidopsis ethylene signaling mutants etr1, ein2, and ein3/eil1 showed an anthocyanin overaccumulation phenotype under nitrogen starvation conditions. The ethylene precursor ACC strongly suppressed anthocyanin accumulation, dependent on ETR1, EIN2, EIN3/EIL1, and, partially, RHD3. In addition, inactivating RHD3 partially reversed the suppressive effect of ETO1 inactivation-evoked endogenous ethylene production on anthocyanin accumulation. The expression of nitrogen starvation-induced anthocyanin biosynthesis genes was negatively regulated by RHD3, but ethylene response genes were positively regulated by RHD3. Wild-type seedlings overexpressing RHD3 showed similar phenotypes to rhd3 mutants, indicating the existence of a fine-tuned relationship between gene expression and function. RHD3 was initially identified as a gene involved in root hair development. This study uncovered a new physiological function of RHD3 in nitrogen starvation-induced anthocyanin accumulation and ethylene homeostasis. [Correction added on 6 August 2015, after first online publication: “RND3” corrected to “RHD3”.]

 

Wang J, Wang Y, Yang J, Ma C, Zhang Y, Ge T, Qi Z, Kang Y (2015) Arabidopsis ROOT HAIR DEFECTIVE3 is involved in nitrogen starvation-induced anthocyanin accumulation. J Integr Plant Biol 57: 708–721 doi: 10.1111/jipb.12320

Key words: Anthocyanin, ethylene signaling pathway, ROOT HAIR DEFECTIVE3

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