J Integr Plant Biol ›› 2015, Vol. 57 ›› Issue (6): 522-533.DOI: 10.1111/jipb.12316

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Molecular cloning and functional analysis of the FLOWERING LOCUS T (FT) homolog GhFT1 from Gossypium hirsutum

Danli Guo1†, Chao Li1†, Rui Dong1, Xiaobo Li2, Xiangwen Xiao2 and Xianzhong Huang1*   

  • 收稿日期:2014-10-22 接受日期:2014-11-24 出版日期:2014-11-27 发布日期:2014-11-27

Molecular cloning and functional analysis of the FLOWERING LOCUS T (FT) homolog GhFT1 from Gossypium hirsutum

Danli Guo1†, Chao Li1†, Rui Dong1, Xiaobo Li2, Xiangwen Xiao2 and Xianzhong Huang1*   

  1. 1Key Laboratory of Agrobiotechnology, College of Life Sciences, Shihezi University, Shihezi, China
    2Key Laboratory of Chemistry of Plant Resources in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, the Chinese Academy of Sciences, Urumqi, China
  • Received:2014-10-22 Accepted:2014-11-24 Online:2014-11-27 Published:2014-11-27
  • About author:†These authors contributed equally to this work.
    *Correspondence: E-mail: xianzhongh106@163.com

摘要： A florigen homologous gene GhFT1 in cotton was characterized, which shares the similar expression pattern with other FT genes but also is expressed in fiber. GhFT1 protein locates both in the cytoplasm and the nucleus. GhFT1 promotes flowering in transgenic arabidopsis and can be used to promote early maturation in cotton breeding.

Abstract:

FLOWERING LOCUS T (FT) encodes a member of the phosphatidylethanolamine-binding protein (PEBP) family that functions as the mobile floral signal, playing an important role in regulating the floral transition in angiosperms. We isolated an FT-homolog (GhFT1) from Gossypium hirsutum L. cultivar, Xinluzao 33 GhFT1 was predominantly expressed in stamens and sepals, and had a relatively higher expression level during the initiation stage of fiber development. GhFT1 mRNA displayed diurnal oscillations in both long-day and short-day condition, suggesting that the expression of this gene may be under the control of the circadian clock. Subcellular analysis revealed that GhFT1 protein located in the cytoplasm and nucleus. Ectopic expression of GhFT1 in transgenic arabidopsis plants resulted in early flowering compared with wild-type plants. In addition, ectopic expression of GhFT1 in arabidopsis ft-10 mutants partially rescued the extremely late flowering phenotype. Finally, several flowering related genes functioning downstream of AtFT were highly upregulated in the 35S::GhFT1 transgenic arabidopsis plants. In summary, GhFT1 is an FT-homologous gene in cotton that regulates flower transition similar to its orthologs in other plant species and thus it may be a candidate target for promoting early maturation in cotton breeding.

 

Guo D, Li C, Dong R, Li X, Xiao X, Huang X (2015) Molecular cloning and functional analysis of the FLOWERING LOCUS T (FT) homolog GhFT1 from Gossypium hirsutum. J Integr Plant Biol 57:522–533 doi: 10.1111/jipb.12316

Key words: Cotton, floral transition, florigen, flowering time, FLOWING LOCUS T

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