J Integr Plant Biol ›› 2015, Vol. 57 ›› Issue (1): 127-135.DOI: 10.1111/jipb.12304

• • 上一篇    

Simplification of vacuole structure during plant cell death triggered by culture filtrates of Erwinia carotovora

Yumi Hirakawa1, Toshihisa Nomura2, Seiichiro Hasezawa1 and Takumi Higaki1*   

  • 收稿日期:2014-08-16 接受日期:2014-10-27 出版日期:2015-01-01 发布日期:2014-10-31

Simplification of vacuole structure during plant cell death triggered by culture filtrates of Erwinia carotovora

Yumi Hirakawa1, Toshihisa Nomura2, Seiichiro Hasezawa1 and Takumi Higaki1*   

  1. 1Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha Kashiwa, Chiba, Japan
    2RIKEN, Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan
  • Received:2014-08-16 Accepted:2014-10-27 Online:2015-01-01 Published:2014-10-31
  • About author:*Correspondence: E-mail: higaki@k.u-tokyo.ac.jp

摘要： In defense-related cell death, vacuole simplification has been considered as a prelude to the vacuole’s rupture. Hirakawa et al. discovered a new type of the cell death that vacuoles become simpler but maintain their integrity at the onset of cell death. The results call for rethinking the roles of vacuole simplification.

Abstract:

Vacuoles are suggested to play crucial roles in plant defense-related cell death. During programmed cell death, previous live cell imaging studies have observed vacuoles to become simpler in structure and have implicated this simplification as a prelude to the vacuole's rupture and consequent lysis of the plasma membrane. Here, we examined dynamics of the vacuole in cell cycle-synchronized tobacco BY-2 (Nicotiana tabacum L. cv. Bright Yellow 2) cells during cell death induced by application of culture filtrates of Erwinia carotovora. The filtrate induced death in about 90% of the cells by 24 h. Prior to cell death, vacuole shape simplified and endoplasmic actin filaments disassembled; however, the vacuoles did not rupture until after plasma membrane integrity was lost. Instead of facilitating rupture, the simplification of vacuole structure might play a role in the retrieval of membrane components needed for defense-related cell death.

 

Hirakawa Y, Nomura T, Hasezawa S, Higaki T (2015) Simplification of vacuole structure during plant cell death triggered by culturefiltrates of Erwinia carotovora. J Integr Plant Biol 57: 127–135. doi: 10.1111/jipb.12304

Key words: Actin filaments, defense-related cell death, Erwinia carotovora, tobacco BY-2 cells, vacuoles

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