J Integr Plant Biol ›› 2023, Vol. 65 ›› Issue (1): 10-24.DOI: 10.1111/jipb.13356

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  • 收稿日期:2022-05-09 接受日期:2022-09-01 出版日期:2023-01-01 发布日期:2023-02-02

Brassinosteroid signaling positively regulates abscisic acid biosynthesis in response to chilling stress in tomato

Shengmin An1, Yue Liu1, Kangqi Sang1, Ting Wang1, Jingquan Yu1,2,3, Yanhong Zhou1,2, Xiaojian Xia1,2*   

  1. 1. Department of Horticulture Zhejiang University Hangzhou 310058 China;
    2. Hainan Institute Zhejiang University Sanya 572025 China;
    3. Key Laboratory of Horticultural Plants Growth, Development and Quality Improvement, Agricultural Ministry of China Hangzhou 310058 China
    *Correspondence: Xiaojian Xia (xiaojianxia@zju.edu.cn)
  • Received:2022-05-09 Accepted:2022-09-01 Online:2023-01-01 Published:2023-02-02

Abstract: Brassinosteroids (BRs) and abscisic acid (ABA) are essential regulators of plant growth and stress tolerance. Although the antagonistic interaction of BRs and ABA is proposed to ensure the balance between growth and defense in model plants, the crosstalk between BRs and ABA in response to chilling in tomato (Solanum lycopersicum), a warm-climate horticultural crop, is unclear. Here, we determined that overexpression of the BR biosynthesis gene DWARF (DWF) or the key BR signaling gene BRASSINAZOLE-RESISTANT1 (BZR1) increases ABA levels in response to chilling stress via positively regulating the expression of the ABA biosynthesis gene 9-CIS-EPOXYCAROTENOID DIOXYGENASE1 (NCED1). BR-induced chilling tolerance was mostly dependent on ABA biosynthesis. Chilling stress or high BR levels decreased the abundance of BRASSINOSTEROID-INSENSITIVE2 (BIN2), a negative regulator of BR signaling. Moreover, we observed that chilling stress increases BR levels and results in the accumulation of BZR1. BIN2 negatively regulated both the accumulation of BZR1 protein and chilling tolerance by suppressing ABA biosynthesis. Our results demonstrate that BR signaling positively regulates chilling tolerance via ABA biosynthesis in tomato. The study has implications in production of warm-climate crops in horticulture.

Key words: ABA, BIN2, BZR1, chilling stress, NCED

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