J Integr Plant Biol ›› 2024, Vol. 66 ›› Issue (4): 660-682.DOI: 10.1111/jipb.13585

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  • 收稿日期:2023-08-31 接受日期:2023-11-14 出版日期:2024-04-01 发布日期:2024-04-13

OsNAC5 orchestrates OsABI5 to fine-tune cold tolerance in rice

Ruiqing Li1†, Yue Song2,3†, Xueqiang Wang2,3†, Chenfan Zheng2,3, Bo Liu2,3, Huali Zhang4, Jian Ke1, Xuejing Wu1, Liquan Wu1*, Ruifang Yang5* and Meng Jiang2,3*   

  1. 1. College of Agronomy, Anhui Agricultural University, Hefei 230036, China;
    2. Hainan Institute, Yazhou Bay Sci-Tech City, Zhejiang University, Sanya 572025, China;
    3. National Key Laboratory of Rice Biology, Advanced Seed Institute, Zhejiang University, Hangzhou 311225, China;
    4. State Key Laboratory of Rice Biology and Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 311401, China;
    5. Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops(Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
    †These Authors contributed equally to this work.
    *Correspondences:Liquan Wu(wlq-001@163.com);Ruifang Yang(yangruifang1982@163.com);Meng Jiang(mengjiang@zju.edu.cn, Dr. Jiang is responsible for the distributions of the materials associated with this article)
  • Received:2023-08-31 Accepted:2023-11-14 Online:2024-04-01 Published:2024-04-13

Abstract: Due to its tropical origins, rice (Oryza sativa) is susceptible to cold stress, which poses severe threats to production. OsNAC5, a NAC-type transcription factor, participates in the cold stress response of rice, but the detailed mechanisms remain poorly understood. Here, we demonstrate that OsNAC5 positively regulates cold tolerance at germination and in seedlings by directly activating the expression of ABSCISIC ACID INSENSITIVE 5 (OsABI5). Haplotype analysis indicated that single nucleotide polymorphisms in a NAC-binding site in the OsABI5 promoter are strongly associated with cold tolerance. OsNAC5 also enhanced OsABI5 stability, thus regulating the expression of cold-responsive (COR) genes, enabling fine-tuned control of OsABI5 action for rapid, precise plant responses to cold stress. DNA affinity purification sequencing coupled with transcriptome deep sequencing identified several OsABI5 target genes involved in COR expression, including DEHYDRATION-RESPONSIVE ELEMENT BINDING FACTOR 1A (OsDREB1A), OsMYB20, and PEROXIDASE 70 (OsPRX70). In vivo and in vitro analyses suggested that OsABI5 positively regulates COR gene transcription, with marked COR upregulation in OsNAC5-overexpressing lines and downregulation in osnac5 and/or osabi5 knockout mutants. This study extends our understanding of cold tolerance regulation via OsNAC5 through the OsABI5-CORs transcription module, which may be used to ameliorate cold tolerance in rice via advanced breeding.

Key words: ABA signaling, ABI5, cold tolerance, DREB, NAC5, rice

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