J Integr Plant Biol ›› 2024, Vol. 66 ›› Issue (8): 1620-1638.DOI: 10.1111/jipb.13709  cstr: 32098.14.jipb.13709

所属专题： Development； SDGs 目标2: 零饥饿

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  • 收稿日期:2023-08-05 修回日期:2024-05-04 出版日期:2024-08-01 发布日期:2024-08-19

GmDFB1, an ARM‐repeat superfamily protein, regulates floral organ identity through repressing siRNA‐ and miRNA‐mediated gene silencing in soybean

Jie Li1, Wenxiao Zhang1, Qing Lu1, Jiaqi Sun1, Chuang Cheng1, Shiyu Huang1, Shuo Li1, Qiang Li1, Wei Zhang1, Chuanen Zhou1, Bin Liu2 and Fengning Xiang1*   

  1. 1. The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao 266237, China
    2. State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    *Correspondence: Fengning Xiang (xfn0990@sdu.edu.cn)
  • Received:2023-08-05 Revised:2024-05-04 Online:2024-08-01 Published:2024-08-19
  • Supported by:
    This research was supported by the National Key Research and Development Program of China (Grant Nos. 2021YFF1001201 and 2022YFF1001601‐4), Key Research and Development Program of Shandong Province (Grant Nos. 2023LZGC008 and 2021LZGC003), the National Scientific and Technological Innovation 2030‐Major Project (Grant No. 2023ZD040360102), the Joint Funds of the National Natural Science Foundation of China (Grant No. U1906203), the National Transgenic Project of China (Grant Nos. 2016ZX08010002‐009 and 2018ZX08009‐14B), Taishan Scholar Youth Project of Shandong Province (Grant No. tsqn202306072), Young Scientists Fund of the National Natural Science Foundation of China (Grant No. 32301876), Natural Science Foundation of Shandong Province Youth Fund (Grant No. ZR2023QC180) and Qingdao Natural Science Foundation (Grant No. 23‐2‐1‐38‐zyyd‐jch), Shandong University Qilu Young Scholar.

Abstract: The development of flowers in soybean (Glycine max) is essential for determining the yield potential of the plant. Gene silencing pathways are involved in modulating flower development, but their full elucidation is still incomplete. Here, we conducted a forward genetic screen and identified an abnormal flower mutant, deformed floral bud1‐1 (Gmdfb1‐1), in soybean. We mapped and identified the causal gene, which encodes a member of the armadillo (ARM)‐repeat superfamily. Using small RNA sequencing (sRNA‐seq), we found an abnormal accumulation of small interfering RNAs (siRNAs) and microRNA (miRNAs) in the Gmdfb1 mutants. We further demonstrated that GmDFB1 interacts with the RNA exosome cofactor SUPER KILLER7 (GmSKI7). Additionally, GmDFB1 interacts with the PIWI domain of ARGONAUTE 1 (GmAGO1) to inhibit the cleavage efficiency on the target genes of sRNAs. The enhanced gene silencing mediated by siRNA and miRNA in the Gmdfb1 mutants leads to the downregulation of their target genes associated with flower development. This study revealed the crucial role of GmDFB1 in regulating floral organ identity in soybean probably by participating in two distinct gene silencing pathways.

Key words: ARM‐repeat superfamily protein, ARGONAUTE 1, SUPER KILLER 7, flower development, gene silencing, small RNAs, soybean

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