J Integr Plant Biol

Getting started in mapping-by-sequencing

Héctor Candela, Rubén Casanova-Sáez, and José Luis Micol

2015, 57(7): 606-612. doi:10.1111/jipb.12305

摘要 ( 3956 )

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Next-generation sequencing technologies allow the rapid mapping and identification of point mutations, a strategy that has been dubbed “mapping-by-sequencing”. We review examples where these technologies have led to the identification of mutations in Arabidopsis and other model species, such as yeast or Drosophila. Mapping-by-sequencing will also help to understand the molecular basis of many traits in species with larger genomes, such as many crops.

CRISPR Primer Designer: Design primers for knockout and chromosome imaging CRISPR-Cas system

Meng Yan, Shi-Rong Zhou, and Hong-Wei Xue

2015, 57(7): 613-617. doi:10.1111/jipb.12295

摘要 ( 4801 )

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CRISPR Primer Designer helps CRISPR-Cas system users to choose specific spacer sequences for genomes editing and chromosome imaging. Not only for plants, but for all organisms that support Whole Genome BLAST in NCBI, local BLAST or other sites. We will update the program continually.

Jasmonic acid-isoleucine formation in grapevine (Vitis vinifera L.) by two enzymes with distinct transcription profiles

Christine Böttcher, Crista A. Burbidge, Valentina di Rienzo, Paul K. Boss and Christopher Davies

2015, 57(7): 618-627. doi:10.1111/jipb.12321

摘要 ( 1961 )

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In grapevine, the hormone JA-Ile was shown to be produced by two enzymes, VvGH3-7 and VvGH3-9. Studies of gene expression and hormone measurements revealed that the above two GH3 proteins are likely to be involved in early berry development, but play different roles in the leaf wound response.

Hydrogen sulfide regulates abiotic stress tolerance and biotic stress resistance in Arabidopsis

Haitao Shi, Tiantian Ye, Ning Han, Hongwu Bian, Xiaodong Liu, and Zhulong Chan

2015, 57(7): 628-640. doi:10.1111/jipb.12302

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H2S is an important gaseous molecule in various plant developmental processes and plant stress responses. Herein, this study shows that cysteine desulfhydrase and H2S confer abiotic stress tolerance and biotic stress resistance in Arabidopsis, via affecting the stress-related gene expressions, ROS metabolism, metabolic homeostasis, and MIR393-targeted auxin receptors.

Enhancement of innate immune system in monocot rice by transferring the dicotyledonous elongation factor Tu receptor EFR

Fen Lu, Huiqin Wang, Shanzhi Wang, Wendi Jiang, Changlin Shan, Bin Li, Jun Yang, Shiyong Zhang, and Wenxian Sun

2015, 57(7): 641-652. doi:10.1111/jipb.12306

摘要 ( 2982 )

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Heterologous expression of EFR, a Brassicaceae-specific pattern recognition receptor, in rice was demonstrated to be functional to sense bacterial EF-Tu and confer broad-spectrum bacterial disease resistance. The study opens new avenues for genetic engineering of broad-spectrum disease resistance in rice by transfer of pattern recognition receptor genes across plant classes.

Effect of elevated CO₂ concentration on photosynthetic characteristics of hyperaccumulator Sedum alfredii under cadmium stress

Tingqiang Li, Qi Tao, Zhenzhen Di, Fan Lu and Xiaoe Yang

2015, 57(7): 653-660. doi:10.1111/jipb.12307

摘要 ( 2232 )

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An increase in atmospheric CO₂ concentration has profound influences on plant growth. In this study, we found that elevated CO₂ promoted the growth of Sedum alfredii, a Cd hyperaccumulator native to China, due to increased photosynthetic carbon uptake rate and photosynthetic light-use efficiency, and significantly increased Cd uptake by S. alfredii.

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