Abstract: Prevacuolar compartments (PVCs) and endosomal compartments aremembrane-bound organellesmediating protein traffic to vacuoles in the secretory and endocytic pathways of plant cells. Over the years, great progress has been made towards our understanding in these two compartments in plant cells. In this review, we will summarize our contributions toward the identification and characterization of plant prevacuolar and endosomal compartments. Our studies will serve as important steps in future molecular characterization of PVC biogenesis and PVC-mediated protein trafficking in plant cells.

Key words: BY-2 cells, endosomal compartment, prevacuolar compartment, organelle proteomic, vacuolar sorting receptor.