]*>","")" /> Phylogenetically Distinct Cellulose Synthase Genes Support Secondary Wall Thickening in Arabidopsis Shoot Trichomes and Cotton Fiber

J Integr Plant Biol ›› 2010, Vol. 52 ›› Issue (2): 205-220.DOI: 10.1111/j.1744-7909.2010.00934.x

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Phylogenetically Distinct Cellulose Synthase Genes Support Secondary Wall Thickening in Arabidopsis Shoot Trichomes and Cotton Fiber

Lissete Betancur1, Bir Singh2, Ryan A. Rapp3, Jonathan F. Wende3, M. David Marks4, Alison W. Roberts5 and Candace H. Haigler1,2*   

  • 收稿日期:2009-08-01 接受日期:2009-12-17 出版日期:2010-02-01 发布日期:2010-02-05

Phylogenetically Distinct Cellulose Synthase Genes Support Secondary Wall Thickening in Arabidopsis Shoot Trichomes and Cotton Fiber

Lissete Betancur1, Bir Singh2, Ryan A. Rapp3, Jonathan F. Wende3, M. David Marks4, Alison W. Roberts5 and Candace H. Haigler1,2*   

  1. 1Department of Plant Biology, North Carolina State University, Box 7612, Raleigh NC 27695-7612 USA
    2Department of Crop Science, North Carolina State University, Box 7620, Raleigh NC 27695-7620 USA
    3EEOB Department, Iowa State University, 251 Bessey Hall, Ames, IA 50011 USA
    4Department of Plant Biology, University of Minnesota, 1445 Gortner Ave, St. Paul MN 55108 USA
    5Department of Biological Sciences, CBLS, University of Rhode Island, Kingston, RI 02881 USA Current Address: Ryan A. Rapp, Department of Molecular Breeding, Monsanto Company, 800 North Lindbergh Blvd., Mail Stop N3SA, St. Louis, MO 63167, USA
  • Received:2009-08-01 Accepted:2009-12-17 Online:2010-02-01 Published:2010-02-05
  • About author:*Author for Correspondence Tel: +1 919-515-5645; FAX: +1 919-515-5315; E-mail: candace_haigler@ncsu.edu

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Abstract:

Through exploring potential analogies between cotton seed trichomes (or cotton fiber) and arabidopsis shoot trichomes we discovered that CesAs from either the primary or secondary wall phylogenetic clades can support secondary wall thickening. CesA genes that typically support primary wall synthesis, AtCesA1,2,3,5, and 6, underpin expansion and secondary wall thickening of arabidopsis shoot trichomes. In contrast, apparent orthologs of CesA genes that support secondary wall synthesis in arabidopsis xylem, AtCesA4,7, and 8, are up-regulated for cotton fiber secondary wall deposition. These conclusions arose from: (a) analyzing the expression of CesA genes in arabidopsis shoot trichomes; (b) observing birefringent secondary walls in arabidopsis shoot trichomes with mutations in AtCesA4, 7, or 8; (c) assaying up-regulated genes during different stages of cotton fiber development; and (d) comparing genes that were co-expressed with primary or secondary wall CesAs in arabidopsis with genes upregulated in arabidopsis trichomes, arabidopsis secondary xylem, or cotton fiber during primary or secondary wall deposition. Cumulatively, the data show that: (a) the xylem of arabidopsis provides the best model for secondary wall cellulose synthesis in cotton fiber; and (b) CesA genes within a “cell wall toolbox” are used in diverse ways for the construction of particular specialized cell walls.

Betancur L, Singh B, Rapp RA, Wendel JF, Marks MD, Roberts AW and Haigler CH (2010) Phylogenetically distinct cellulose synthase genes support secondary wall thickening in arabidopsis shoot trichomes and cotton fiber. J. Integr. Plant Biol. 52(2), 205–220.

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