]*>","")" /> Expression Profiling of Cassava Storage Roots Reveals an Active Process of Glycolysis/Gluconeogenesis

J Integr Plant Biol ›› 2011, Vol. 53 ›› Issue (3): 193-211.DOI: 10.1111/j.1744-7909.2010.01018.x

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Expression Profiling of Cassava Storage Roots Reveals an Active Process of Glycolysis/Gluconeogenesis

Jun Yang1, Dong An2 and Peng Zhang1,2,3*   

  • 收稿日期:2010-11-25 接受日期:2010-12-01 出版日期:2011-03-01 发布日期:2010-12-07

Expression Profiling of Cassava Storage Roots Reveals an Active Process of Glycolysis/Gluconeogenesis

Jun Yang1, Dong An2 and Peng Zhang1,2,3*   

  1. 1Shanghai Center for Cassava Biotechnology, National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology & Ecology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200032, China
    2Key Laboratory of Synthetic Biology, Institute of Plant Physiology & Ecology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200032, China
    3Shanghai Chenshan Plant Science Research Center,the Chinese Academy of Sciences, Chenshan Botanical Garden, Shanghai, 201602, China
  • Received:2010-11-25 Accepted:2010-12-01 Online:2011-03-01 Published:2010-12-07
  • About author:
    *Corresponding author Tel: +86 21 5492 4096; Fax: +86 21 5492 4318; E-mail: zhangpeng@sibs.ac.cn

被引次数

90

Abstract:

Mechanisms related to the development of cassava storage roots and starch accumulation remain largely unknown. To evaluate genome-wide expression patterns during tuberization, a 60 mer oligonucleotide microarray representing 20 840 cassava genes was designed to identify differentially expressed transcripts in fibrous roots, developing storage roots and mature storage roots. Using a random variance model and the traditional twofold change method for statistical analysis, 912 and 3 386 upregulated and downregulated genes related to the three developmental phases were identified. Among 25 significantly changed pathways identified, glycolysis/gluconeogenesis was the most evident one. Rate-limiting enzymes were identified from each individual pathway, for example, enolase, L-lactate dehydrogenase and aldehyde dehydrogenase for glycolysis/gluconeogenesis, and ADP-glucose pyrophosphorylase, starch branching enzyme and glucan phosphorylase for sucrose and starch metabolism. This study revealed that dynamic changes in at least 16% of the total transcripts, including transcription factors, oxidoreductases/transferases/hydrolases, hormone-related genes, and effectors of homeostasis. The reliability of these differentially expressed genes was verified by quantitative real-time reverse transcription-polymerase chain reaction. These studies should facilitate our understanding of the storage root formation and cassava improvement.

Yang J, An D, Zhang P (2011) Expression profiling of cassava storage roots reveals an active process of glycolysis/gluconeogenesis. J. Integr. Plant Biol. 53(3), 193–211.

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