]*>","")" /> Transferability of Microsatellite Markers from <i>Brachypodium distachyon</i> to <i>Miscanthus sinensis</i>, a Potential Biomass Crop F

J Integr Plant Biol ›› 2011, Vol. 53 ›› Issue (3): 232-245.DOI: 10.1111/j.1744-7909.2010.01026.x

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Transferability of Microsatellite Markers from Brachypodium distachyon to Miscanthus sinensis, a Potential Biomass Crop F

Hua Zhao1, Jiangyan Yu1, Fank M. You2, Mingcheng Luo2 and Junhua Peng1,3**   

  • 收稿日期:2010-10-09 接受日期:2010-12-14 出版日期:2011-03-01 发布日期:2010-12-22

Transferability of Microsatellite Markers from Brachypodium distachyon to Miscanthus sinensis, a Potential Biomass Crop F

Hua Zhao1, Jiangyan Yu1, Fank M. You2, Mingcheng Luo2 and Junhua Peng1,3**   

  1. 1Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, and Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China
    2Department of Plant Sciences, University of California, 265 Hunt Hall, One Shields Ave., Davis, CA 95616, USA
    3Department of Soil and Crop Sciences, Colorado State University, Fort Collins, CO 80523-1170, USA

Abstract:

Miscanthus sinensis has high biomass yield and contributed two of the three genomes in M. x giganteus, a bioenergy crop widely studied in Europe and North America, and thus is a potential biomass crop and an important germplasm for Miscanthus breeding. Molecular markers are essential for germplasm evaluation, genetic analyses and new cultivar development in M. sinensis. In the present study, we reported transferability of simple sequence repeat (SSR) markers from Brachypodium distachyon to M. sinensis. A set of 57 SSR markers evenly distributed across the B. distachyon genome were deliberately designed. Out of these B. distachyon SSR markers, 86.0% are transferable to M. sinensis. The SSR loci amplified in M. sinensis were validated by re-sequencing the amplicons. The polymorphism information content (PIC) of the transferable SSR markers varied from 0.073 to 0.375 with a mean of 0.263, assessed based on 21 M. sinensis genotypes. Phylogenetic tree based on 162 alleles detected by 49 SSR markers could unambiguously distinguish B. distachyon from M. sinensis, and cluster 21 M. sinensis genotypes into three groups that are basically in coincidence with their geographical distribution and ecotype classifications. The markers developed by the comparative genomic approach could be useful for germplasm evaluation, genetic analysis, and marker-assisted breeding in Miscanthus.

Zhao H, Yu J, You FM, Luo M, Peng JH (2011) Transferability of microsatellite markers from Brachypodium distachyon to Miscanthus sinensis, a potential biomass crop. J. Integr. Plant Biol. 53(3), 232–245.

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