J Integr Plant Biol ›› 2015, Vol. 57 ›› Issue (5): 496-503.DOI: 10.1111/jipb.12322

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A one-step rectification of sperm cell targeting ensures the success of double fertilization

Jilei Huang1,2, Yan Ju1†, Xiangfeng Wang1, Quan Zhang1 and Sodmergen1*   

  • 收稿日期:2014-08-09 接受日期:2014-12-17 出版日期:2014-12-22 发布日期:2014-12-22

A one-step rectification of sperm cell targeting ensures the success of double fertilization

Jilei Huang1,2, Yan Ju1†, Xiangfeng Wang1, Quan Zhang1 and Sodmergen1*   

  1. 1Key Laboratory of Cell Proliferation and Differentiation (Ministry of Education), College of Life Sciences, Peking University, Beijing, China
    2Instrumental Analysis and Research Center, South China Agricultural University, Guangzhou, China
  • Received:2014-08-09 Accepted:2014-12-17 Online:2014-12-22 Published:2014-12-22
  • About author:†These authors contributed equally to this work.
    *Correspondence: E-mail: sodmergn@pku.edu.cn

被引次数

2

摘要： It is largely a mystery that how immobile sperm cells of flowering plants get into fusions with the egg and central cells. Through live-cell imaging of the embryo sac, we show a dramatic release and re-adhesion of one sperm cell, modifying gamete associations and ensuring the success of double fertilization.

Abstract:

Successful fertilization in animals depends on competition among millions of sperm cells, whereas double fertilization in flowering plants usually involves just one pollen tube releasing two immobile sperm cells. It is largely a mystery how the plant sperm cells fuse efficiently with their female targets within an embryo sac. We show that the initial positioning of sperm cells upon discharge from the pollen tube is usually inopportune for gamete fusions and that adjustment of sperm cell targeting occurs through release and re-adhesion of one sperm cell, while the other connected sperm cell remains in stagnation. This enables proper adhesion of each sperm cell to a female gamete and coordinates the gamete fusions. Our findings reveal inner embryo sac dynamics that ensure the reproductive success of flowering plants and suggest a requirement for sperm cell differentiation as the basis of double fertilization.

 

Huang J, Ju Y, Wang X, Zhang Q, Sodmergen (2015) A one-step rectification of sperm cell targeting ensures the success of double fertilization. J Integr Plant Biol 57: 496–503 doi: 10.1111/jipb.12322

Key words: Arabidopsis, double fertilization, live image, sperm cell targeting

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