J Integr Plant Biol ›› 2015, Vol. 57 ›› Issue (12): 1031-1045.DOI: 10.1111/jipb.12328

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Differential transcriptome analysis between Populus and its synthesized allotriploids driven by second-division restitution

Shiping Cheng1,2, Zhen Huang1,2, Yun Li1,2, Ting Liao1,2, Yujing Suo1,2, Pingdong Zhang1,2, Jun Wang1,2 and Xiangyang Kang1,2*   

  • 收稿日期:2014-09-28 接受日期:2014-12-23 出版日期:2014-12-30 发布日期:2014-12-30

Differential transcriptome analysis between Populus and its synthesized allotriploids driven by second-division restitution

Shiping Cheng1,2, Zhen Huang1,2, Yun Li1,2, Ting Liao1,2, Yujing Suo1,2, Pingdong Zhang1,2, Jun Wang1,2 and Xiangyang Kang1,2*   

  1. 1National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
    2Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
  • Received:2014-09-28 Accepted:2014-12-23 Online:2014-12-30 Published:2014-12-30
  • About author:*Correspondence: E-mail: kangxy@bjfu.edu.cn

摘要： Populus allotriploid has considerable effects on duplicate gene expression, and these genes participate in apical growth. Additionally, triploid hybrids exhibiting high-and low-growth phenotypes differ in terms of gene expression.

Abstract:

In this report, we compared transcriptomic differences between a synthetic Populus section Tacamahaca triploid driven by second-division restitution and its parents using a high-throughput RNA-seq method. A total of 4,080 genes were differentially expressed between the high-growth vigor allotriploids (SDR-H) and their parents, and 719 genes were non-additively expressed in SDR-H. Differences in gene expression between the allotriploid and male parent were more significant than those between the allotriploid and female parent, which may be caused by maternal effects. We observed 3,559 differentially expressed genes (DEGs) between the SDR-H and male parent. Notably, the genes were mainly involved in metabolic process, cell proliferation, DNA methylation, cell division, and meristem and developmental growth. Among the 1,056 DEGs between SDR-H and female parent, many genes were associated with metabolic process and carbon utilization. In addition, 1,789 DEGs between high- and low-growth vigor allotriploid were mainly associated with metabolic process, auxin poplar transport, and regulation of meristem growth. Our results indicated that the higher poplar ploidy level can generate extensive transcriptomic diversity compared with its parents. Overall, these results increased our understanding of the driving force for phenotypic variation and adaptation in allopolyploids driven by second-division restitution.

 

Cheng S, Huang Z, Li Y, Liao T, Suo Y, Zhang P, Wang J, Kang X (2015) Differential transcriptome analysis between Populus and its synthesized allotriploids driven by second-division restitution. J Integr Plant Biol 57: 1031–1045 doi: 10.1111/jipb.12328

Key words: High-growth vigor, poplar allotriploid, RNA-seq, second-division restitution, transcriptome

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