J Integr Plant Biol ›› 2015, Vol. 57 ›› Issue (4): 411-428.DOI: 10.1111/jipb.12337
Lenka Franková and Stephen C. Fry
Lenka Franková and Stephen C. Fry*
摘要: We demonstrate a novel glass fiber blotting method for assaying transglycanases – enzymes that cut and paste polysaccharides. Compared with conventional methods, also discussed in this paper, it allows screening of non-desalted enzyme extracts for multiple proposed enzyme activities utilizing a wide range of qualitatively different polysaccharide and oligosaccharide substrates.