J Integr Plant Biol ›› 2015, Vol. 57 ›› Issue (11): 936-942.DOI: 10.1111/jipb.12355

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An ACCUMULATION AND REPLICATION OF CHLOROPLASTS 5 gene mutation confers light green peel in cucumber

Qian Zhou1,2†, Shenhao Wang1,2†, Bowen Hu1,2†, Huiming Chen3, Zhonghua Zhang1* and Sanwen Huang1,2*   

  • 收稿日期:2014-11-02 接受日期:2015-03-23 出版日期:2015-11-01 发布日期:2015-03-26

An ACCUMULATION AND REPLICATION OF CHLOROPLASTS 5 gene mutation confers light green peel in cucumber

Qian Zhou1,2†, Shenhao Wang1,2†, Bowen Hu1,2†, Huiming Chen3, Zhonghua Zhang1* and Sanwen Huang1,2*   

  1. 1Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture, Sino-Dutch Joint Laboratory of Horticultural Genomics, Beijing, China
    2Agricultural Genomic Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China
    3Hunan Vegetable Research Institute, Hunan Academy of Agricultural Science, Changsha, China
  • Received:2014-11-02 Accepted:2015-03-23 Online:2015-11-01 Published:2015-03-26
  • About author:†These authors contributed equally to this work.
    **Correspondences: E-mail: huangsanwen@caas.cn; zhangzhonghua@caas.cn

摘要： We discovered a cucumber EMS mutant showing distinct light green exocarp. By combining whole genome sequencing with genetic mapping, we identified Csa7G051430, a homolog of Arabidopsis ARC5, as the candidate gene responsible for the altered peel color. The mutation caused abnormal chloroplast development in exocarp cells.

Abstract:

The peel color of fruit is an important commercial trait in cucumber, but the underlying molecular basis is largely unknown. A mutant showing light green exocarp was discovered from ethyl methane sulfonate (EMS) mutagenized cucumber line 406 with dark green exocarp. Genetic analysis showed the mutant phenotype is conferred by a single recessive gene, here designated as lgp (light green peel). By re-sequencing of bulked segregants, we identified the candidate gene Csa7G051430 encoding ACCUMULATION AND REPLICATION OF CHLOROPLASTS 5 (ARC5) that plays a vital role in chloroplast division in Arabidopsis. A single nucleotide polymorphism (SNP) causing amino acid alteration in the conserved GTPase domain of Csa7G051430 showed co-segregation with the altered phenotype. Furthermore, the transient RNA interference of this gene resulted in reduced number and enlarged size of chloroplasts, which were also observed in the lgp mutant. This evidence supports that the non-synonymous SNP in Csa7G051430 is the causative mutation for the light green peel. This study provides a new allele for cucumber breeding for light green fruits and additional resource for the study of chloroplast development.

 

Zhou Q, Wang S, Hu B, Chen H, Zhang Z, Huang S (2015) An ACCUMULATION AND REPLICATION OF CHLOROPLASTS 5 gene mutation confers light green peel in cucumber. J Integr Plant Biol 57: 936–942 doi: 10.1111/jipb.12355

Key words: ARC5, Cucumis sativus, ethyl methane sulfonate mutant, fruit peel color, whole genome re-sequencing

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