J Integr Plant Biol ›› 2016, Vol. 58 ›› Issue (1): 81-90.DOI: 10.1111/jipb.12358

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Identification and fine mapping of quantitative trait loci for the number of vascular bundle in maize stem

Cheng Huang†, Qiuyue Chen†, Guanghui Xu, Dingyi Xu, Jinge Tian and Feng Tian*   

  • 收稿日期:2015-01-04 接受日期:2015-04-02 出版日期:2015-04-03 发布日期:2015-04-03

Identification and fine mapping of quantitative trait loci for the number of vascular bundle in maize stem

Cheng Huang†, Qiuyue Chen†, Guanghui Xu, Dingyi Xu, Jinge Tian and Feng Tian*   

  1. Department of Plant Genetics and Breeding, National Maize Improvement Center of China, China Agricultural University, Beijing, China
  • Received:2015-01-04 Accepted:2015-04-02 Online:2015-04-03 Published:2015-04-03
  • About author:†These authors contributed equally to this work.
    *Correspondence: E-mail: ft55@cau.edu.cn

被引次数

37

摘要： Vascular bundle is the plant transport system that links source and sink, and acts as an important factor for yield potential realization. We report a high-resolution QTL mapping for vascular bundle number in maize stem using a large maize-teosinte population, and show that vascular bundle number is dominated by a large number of small-effect QTLs.

Abstract:

Studies that investigated the genetic basis of source and sink related traits have been widely conducted. However, the vascular system that links source and sink received much less attention. When maize was domesticated from its wild ancestor, teosinte, the external morphology has changed dramatically; however, less is known for the internal anatomy changes. In this study, using a large maize-teosinte experimental population, we performed a high-resolution quantitative trait locus (QTL) mapping for the number of vascular bundle in the uppermost internode of maize stem. The results showed that vascular bundle number is dominated by a large number of small-effect QTLs, in which a total of 16 QTLs that jointly accounts for 52.2% of phenotypic variation were detected, with no single QTL explaining more than 6% of variation. Different from QTLs for typical domestication traits, QTLs for vascular bundle number might not be under directional selection following domestication. Using Near Isogenic Lines (NILs) developed from heterogeneous inbred family (HIF), we further validated the effect of one QTL qVb9-2 on chromosome 9 and fine mapped the QTL to a 1.8-Mb physical region. This study provides important insights for the genetic architecture of vascular bundle number in maize stem and sets basis for cloning of qVb9-2.

 

Huang C, Chen Q, Xu G, Xu D, Tian J, Tian F (2016) Identification and fine mapping of quantitative trait loci for the number of vascular bundle in maize stem. J Integr Plant Biol 58: 81–90 doi: 10.1111/jipb.12358

Key words: Fine-mapping, genetic architecture, maize, quantitative trait locus, vascular bundle number

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