J Integr Plant Biol ›› 2016, Vol. 58 ›› Issue (6): 564-576.DOI: 10.1111/jipb.12436

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Jasmonic acid carboxyl methyltransferase regulates development and herbivory-induced defense response in rice

Jinfeng Qi1,2†, Jiancai Li1†, Xiu Han1†, Ran Li1, Jianqiang Wu2, Haixin Yu1, Lingfei Hu1, Yutao Xiao1, Jing Lu1 and Yonggen Lou1*   

  • 收稿日期:2015-06-03 接受日期:2015-10-12 出版日期:2015-10-15 发布日期:2015-10-15

Jasmonic acid carboxyl methyltransferase regulates development and herbivory-induced defense response in rice

Jinfeng Qi1,2†, Jiancai Li1†, Xiu Han1†, Ran Li1, Jianqiang Wu2, Haixin Yu1, Lingfei Hu1, Yutao Xiao1, Jing Lu1 and Yonggen Lou1*   

  1. 1State Key Laboratory of Rice Biology, Institute of Insect Science, Zhejiang University, Hangzhou 310058, China
    2Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
  • Received:2015-06-03 Accepted:2015-10-12 Online:2015-10-15 Published:2015-10-15
  • About author:†These authors contributed equally to this work.
    *Correspondence: E-mail: yglou@zju.edu.cn

被引次数

30

摘要： Jasmonic acid (JA) and related metabolites play key roles in plant defense and growth. Our study shows that a JA carboxyl methyltransferase, by methylating JA to methyl jasmonate, alters levels of JA and related metabolites and thereby regulates plant development as well as herbivore-induced defense responses in rice.

Abstract:

Jasmonic acid (JA) and related metabolites play a key role in plant defense and growth. JA carboxyl methyltransferase (JMT) may be involved in plant defense and development by methylating JA to methyl jasmonate (MeJA) and thus influencing the concentrations of JA and related metabolites. However, no JMT gene has been well characterized in monocotyledon defense and development at the molecular level. After we cloned a rice JMT gene, OsJMT1, whose encoding protein was localized in the cytosol, we found that the recombinant OsJMT1 protein catalyzed JA to MeJA. OsJMT1 is up-regulated in response to infestation with the brown planthopper (BPH; Nilaparvata lugens). Plants in which OsJMT1 had been overexpressed (oe-JMT plants) showed reduced height and yield. These oe-JMT plants also exhibited increased MeJA levels but reduced levels of herbivore-induced JA and jasmonoyl-isoleucine (JA-Ile). The oe-JMT plants were more attractive to BPH female adults but showed increased resistance to BPH nymphs, probably owing to the different responses of BPH female adults and nymphs to the changes in levels of H2O2 and MeJA in oe-JMT plants. These results indicate that OsJMT1, by altering levels of JA and related metabolites, plays a role in regulating plant development and herbivore-induced defense responses in rice.

Key words: Herbivore-induced plant defense, jasmonic acid, jasmonic acid carboxyl methyltransferase, jasmonoyl-isoleucine, methyl jasmonate, Nilaparvata lugens, rice

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