An exon skipping in a SEPALLATA-Like gene is associated with perturbed floral and fruits development in cucumber

doi:10.1111/jipb.12472

J Integr Plant Biol ›› 2016, Vol. 58 ›› Issue (9): 766-771.DOI: 10.1111/jipb.12472

An exon skipping in a SEPALLATA-Like gene is associated with perturbed floral and fruits development in cucumber

Xin Wang^1,2,†, Dongli Gao^2,3,†, Jinjing Sun², Min Liu², Yaoyao Lun², Jianshu Zheng³, Shenhao Wang², Qingzhi Cui⁴, Xiaofeng Wang^1* and Sanwen Huang^1,2*

收稿日期:2015-12-21 接受日期:2016-02-25 出版日期:2016-03-03 发布日期:2016-03-03

An exon skipping in a SEPALLATA-Like gene is associated with perturbed floral and fruits development in cucumber

Xin Wang^1,2,†, Dongli Gao^2,3,†, Jinjing Sun², Min Liu², Yaoyao Lun², Jianshu Zheng³, Shenhao Wang², Qingzhi Cui⁴, Xiaofeng Wang^1* and Sanwen Huang^1,2*

¹State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, China
²Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
³Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
⁴Vegetable Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China

Received:2015-12-21 Accepted:2016-02-25 Online:2016-03-03 Published:2016-03-03
About author:^†These authors contributed equally to the work.
^**Correspondences: E-mail:wangxff99@nwsuaf.edu.cn; huangsanwen@caas.cn

摘要/Abstract

摘要： We isolated a mutant showing perturbations in the development of floral organs and fruits in cucumber. We further identified the causative variant occurring in CsSEP2 by whole genome re-sequencing of the mutant. The variant caused the skipping of exon 6 and abolishment of the transcriptional activity, thus perturbing floral organs and fruits development.

Abstract:

We isolated a mutant showing perturbations in the development of male and female floral organs and fruits. Analysis of the single nucleotide polymorphisms from bulked F₂ pools identified the causative variant occurring in Csa4G126690. Csa4G126690 shows high homology to Arabidopsis SEPALLATA2 (SEP2) thus being designated CsSEP2. The causative variant was located on the splicing site of CsSEP2, resulting in the skipping of exon 6 and abolishment of the transcriptional activity. Our data suggest that CsSEP2 is involved in the floral organ and fruits development by conferring transcriptional activity.

Key words: Cucumis sativus L, EMS mutant, exon skipping, floral and fruits development, whole genome re-sequencing

Xin Wang, Dongli Gao, Jinjing Sun, Min Liu, Yaoyao Lun, Jianshu Zheng, Shenhao Wang, Qingzhi Cui, Xiaofeng Wang, and Sanwen Huang. An exon skipping in a SEPALLATA-Like gene is associated with perturbed floral and fruits development in cucumber[J]. J Integr Plant Biol, 2016, 58(9): 766-771.

An exon skipping in a SEPALLATA-Like gene is associated with perturbed floral and fruits development in cucumber

An exon skipping in a SEPALLATA-Like gene is associated with perturbed floral and fruits development in cucumber

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics

本文评价