J Integr Plant Biol ›› 2016, Vol. 58 ›› Issue (12): 959-970.DOI: 10.1111/jipb.12485

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Transcription factors AS1 and AS2 interact with LHP1 to repress KNOX genes in Arabidopsis

Zhongfei Li1†, Bin Li1†, Jian Liu2, Zhihao Guo1, Yuhao Liu1, Yan Li3, Wen-Hui Shen1,4, Ying Huang3, Hai Huang2, Yijing Zhang2,* and Aiwu Dong1,*   

  • 收稿日期:2016-03-28 接受日期:2016-05-31 出版日期:2016-07-20 发布日期:2016-07-20

Transcription factors AS1 and AS2 interact with LHP1 to repress KNOX genes in Arabidopsis

Zhongfei Li1†, Bin Li1†, Jian Liu2, Zhihao Guo1, Yuhao Liu1, Yan Li3, Wen-Hui Shen1,4, Ying Huang3, Hai Huang2, Yijing Zhang2,* and Aiwu Dong1,*   

  1. 1State Key Laboratory of Genetic Engineering, International Associated Laboratory of CNRS-Fudan-HUNAU on Plant Epigenome Research, Collaborative Innovation Center for Genetics and Development, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, China
    2National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
    3National Center for Protein Science Shanghai, State Key Laboratory of Molecular Biology, Shanghai State Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
    4Institut de Biologie Moléculaire des Plantes du CNRS, Université de Strasbourg, 12 rue du Général Zimmer, Strasbourg Cédex, France
  • Received:2016-03-28 Accepted:2016-05-31 Online:2016-07-20 Published:2016-07-20
  • About author:†These authors contributed equally to this work.
    ***Correspondances: E-mail: aiwudong@fudan.edu.cn(Dr. Aiwu Dong is fully responsible for distributions of all materials associated with this article);zhangyijing@sibs.ac.cn

摘要： The transcription factors ASYMMETRIC LEAVES 1 (AS1) and AS2 directly interact with LIKE HETEROCHROMATIN PROTEIN 1 (LHP1), a reader protein of H3K27me3, and repress the expression of Class-I KNOX genes BP and KNAT2 via establishment of H3K27me3 modification during leaf development in Arabidopsis.

Abstract:

Polycomb group proteins are important repressors of numerous genes in higher eukaryotes. However, the mechanism by which Polycomb group proteins are recruited to specific genes is poorly understood. In Arabidopsis, LIKE HETEROCHROMATIN PROTEIN 1 (LHP1), also known as TERMINAL FLOWER 2, was originally proposed as a subunit of polycomb repressive complex 1 (PRC1) that could bind the tri-methylated lysine 27 of histone H3 (H3K27me3) established by the PRC2. In this work, we show that LHP1 mainly functions with PRC2 to establish H3K27me3, but not with PRC1 to catalyze monoubiquitination at lysine 119 of histone H2A. Our results show that complexes of the transcription factors ASYMMETRIC LEAVES 1 (AS1) and AS2 could help to establish the H3K27me3 modification at the chromatin regions of Class-I KNOTTED1-like homeobox (KNOX) genes BREVIPEDICELLUS and KNAT2 via direct interactions with LHP1. Additionally, our transcriptome analysis indicated that there are probably more common target genes of AS1 and LHP1 besides Class-I KNOX genes during leaf development in Arabidopsis.

Key words: Arabidopsis thaliana, ASYMMETRIC LEAVES 1, ASYMMETRIC LEAVES 2, LIKE HETEROCHROMATIN PROTEIN 1, H3K27me3 establishment, Class-I KNOX genes

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