J Integr Plant Biol ›› 2017, Vol. 59 ›› Issue (4): 275-287.DOI: 10.1111/jipb.12524

• • 上一篇    

ORA59 and EIN3 interaction couples jasmonate-ethylene synergistic action to antagonistic salicylic acid regulation of PDF expression

Xiang He†,‡, Jishan Jiang†, Changquan Wang§ and Katayoon Dehesh*   

  • 收稿日期:2017-01-26 接受日期:2017-02-06 出版日期:2017-02-07 发布日期:2017-02-07

ORA59 and EIN3 interaction couples jasmonate-ethylene synergistic action to antagonistic salicylic acid regulation of PDF expression

Xiang He†,‡, Jishan Jiang†, Changquan Wang§ and Katayoon Dehesh*   

  1. Department of Botany and Plant Sciences and Institute of Integrative Genome Biology, University of California, Riverside, California 92506, USA
  • Received:2017-01-26 Accepted:2017-02-06 Online:2017-02-07 Published:2017-02-07
  • About author:†These authors have contributed equally to the manuscript
    ‡Present address: Rice Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
    §Present address: Department of Ornamental Horticulture, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
    *Correspondence: E-mail: Katayoon Dehesh (katayoon.dehesh@ucr.edu)

被引次数

9

摘要： SA treatment promotes EIN3 binding and targeting to ORA59 for degradation, whereas JA promotes EIN3 transactivation of ORA59 and induction of PDF1.2 Thus ORA59 acts as a coordinating node for the antagonistic function of ET/JA and SA.

Abstract:

Hormonal crosstalk is central for tailoring plant responses to the nature of challenges encountered. The role of antagonism between the two major defense hormones, salicylic acid (SA) and jasmonic acid (JA), and modulation of this interplay by ethylene (ET) in favor of JA signaling pathway in plant stress responses is well recognized, but the underlying mechanism is not fully understood. Here, we show the opposing function of two transcription factors, ethylene insensitive3 (EIN3) and EIN3-Like1 (EIL1), in SA-mediated suppression and JA-mediated activation of PLANT DEFENSIN1.2 (PDF1.2). This functional duality is mediated via their effect on protein, not transcript levels of the PDF1.2 transcriptional activator octadecanoid-responsive Arabidopsis59 (ORA59). Specifically, JA induces ORA59 protein levels independently of EIN3/EIL1, whereas SA reduces the protein levels dependently of EIN3/EIL1. Co-infiltration assays revealed nuclear co-localization of ORA59 and EIN3, and split-luciferase together with yeast-two-hybrid assays established their physical interaction. The functional ramification of the physical interaction is EIN3-dependent degradation of ORA59 by the 26S proteasome.

These findings allude to SA-responsive reduction of ORA59 levels mediated by EIN3 binding to and targeting of ORA59 for degradation, thus nominating ORA59 pool as a coordination node for the antagonistic function of ET/JA and SA.

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