J Integr Plant Biol ›› 2018, Vol. 60 ›› Issue (2): 130-143.DOI: 10.1111/jipb.12557

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OsIDD2, a zinc finger and INDETERMINATE DOMAIN protein, regulates secondary cell wall formation

Peng Huang1,2†, Hideki Yoshida1†‡*, Kenji Yano3, Shunsuke Kinoshita1, Kyosuke Kawai1, Eriko Koketsu1, Masako Hattori1, Sayaka Takehara1, Ji Huang2, Ko Hirano1, Reynante Lacsamana Ordonio4, Makoto Matsuoka1* and Miyako Ueguchi-Tanaka1*   

  • 收稿日期:2017-02-08 接受日期:2017-05-29 出版日期:2018-02-13 发布日期:2017-06-02

OsIDD2, a zinc finger and INDETERMINATE DOMAIN protein, regulates secondary cell wall formation

Peng Huang1,2†, Hideki Yoshida1†‡*, Kenji Yano3, Shunsuke Kinoshita1, Kyosuke Kawai1, Eriko Koketsu1, Masako Hattori1, Sayaka Takehara1, Ji Huang2, Ko Hirano1, Reynante Lacsamana Ordonio4, Makoto Matsuoka1* and Miyako Ueguchi-Tanaka1*   

  1. 1Bioscience and Biotechnology Center, Nagoya University, Nagoya 464-8601, Japan
    2State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
    3Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
    4Plant Breeding and Biotechnology Division, Philippine Rice Research Institute, Maligaya, Science City of Munoz 3119, The Philippines
  • Received:2017-02-08 Accepted:2017-05-29 Online:2018-02-13 Published:2017-06-02
  • About author:†These authors contributed equally to this work
    ‡Current address: Kihara Institute for Biological Research, Yokohama City University, Yokohama 244-0813, Japan
    ***Correspondances: E-mail: Hideki Yoshida (yoshidah@yokohama-cu.ac.jp, Dr. Yoshida is fully responsible for the distribution of all materials associated with this article); Makoto Matsuoka (makoto@nuagr1.agr.nagoya-u.ac.jp); Miyako Ueguchi-Tanaka (mueguchi@nuagr1.agr.nagoya-u.ac.jp)

摘要： Secondary cell wall, which plants make after maturing to reinforce the primary cell wall, is known to be under control of several transcription factors. We added a member of the indeterminate domain family, OsIDD2, to the list of the factors as a negative regulator of lignin biosynthesis in rice.

Abstract:

Previously, we found 123 transcription factors (TFs) as candidate regulators of secondary cell wall (SCW) formation in rice by using phylogenetic and co-expression network analyses. Among them, we examined in this work the role of OsIDD2, a zinc finger and indeterminate domain (IDD) family TF. Its overexpressors showed dwarfism, fragile leaves, and decreased lignin content, which are typical phenotypes of plants defective in SCW formation, whereas its knockout plants showed slightly increased lignin content. The RNA-seq and quantitative reverse transcription polymerase chain reaction analyses confirmed that some lignin biosynthetic genes were downregulated in the OsIDD2-overexpressing plants, and revealed the same case for other genes involved in cellulose synthesis and sucrose metabolism. The transient expression assay using rice protoplasts revealed that OsIDD2 negatively regulates the transcription of genes involved in lignin biosynthesis, cinnamyl alcohol dehydrogenase 2 and 3 (CAD2 and 3), and sucrose metabolism, sucrose synthase 5 (SUS5), whereas an AlphaScreen assay, which can detect the interaction between TFs and their target DNA sequences, directly confirmed the interaction between OsIDD2 and the target sequences located in the promoter regions of CAD2 and CAD3. Based on these observations, we conclude that OsIDD2 is negatively involved in SCW formation and other biological events by downregulating its target genes.

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