J Integr Plant Biol ›› 2021, Vol. 63 ›› Issue (1): 3-33.DOI: 10.1111/jipb.13063

所属专题： Crop yield； Epigenetics； Genome editing

• •    下一篇

  

  • 收稿日期:2020-12-05 接受日期:2020-12-22 出版日期:2021-01-01 发布日期:2021-01-28

Genome editing for plant research and crop improvement

Xiangqiang Zhan1 , Yuming Lu2 , Jian-Kang Zhu2, 3* and Jose Ramon Botella4*   

  1. 1State Key Laboratory of Crop Stress Biology for Arid Areas and College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China
    2Shanghai Center for Plant Stress Biology, CAS Center of Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China
    3Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA
    4School of Agriculture and Food Sciences, University of Queensland, Brisbane, QLD 4072, Australia

    *Correspondences: Jose Ramon Botella (j.botella@uq.edu.au, Dr. Botella is fully responsible for the distributions of all materials associated with this article); Jian-Kang Zhu (jkzhu@psc.ac.cn)
  • Received:2020-12-05 Accepted:2020-12-22 Online:2021-01-01 Published:2021-01-28

Abstract: The advent of clustered regularly interspaced short palindromic repeat (CRISPR) has had a profound impact on plant biology, and crop improvement. In this review, we summarize the state‐of‐the‐art development of CRISPR technologies and their applications in plants, from the initial introduction of random small indel (insertion or deletion) mutations at target genomic loci to precision editing such as base editing, prime editing and gene targeting. We describe advances in the use of class 2, types II, V, and VI systems for gene disruption as well as for precise sequence alterations, gene transcription, and epigenome control.

[an error occurred while processing this directive]