J Integr Plant Biol ›› 2022, Vol. 64 ›› Issue (6): 1281-1294.DOI: 10.1111/jipb.13244

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  • 收稿日期:2022-01-09 接受日期:2022-03-02 出版日期:2022-06-01 发布日期:2022-06-15

Establishment of a dmp based maternal haploid induction system for polyploid Brassica napus and Nicotiana tabacum

Yu Zhong1†, Yuwen Wang1†, Baojian Chen2†, Jinchu Liu1, Dong Wang1, Mengran Li1, Xiaolong Qi1, Chenxu Liu1*, Kim Boutilier2* and Shaojiang Chen1*   

  1. 1 National Maize Improvement Center of China, Key Laboratory of Crop Heterosis and Utilization/Engineering Research Center for Maize Breeding, Ministry of Education, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China
    2 Bioscience, Wageningen University and Research, 6700 AA, Wageningen, The Netherlands

    †These authors contributed equally to this work.
    *Correspondences: Chenxu Liu (liucx@cau.edu.cn); Kim Boutilier (kim.boutilier@wur.nl); Shaojiang Chen (chen368@126.com, Dr. Chen is fully responsible for the distribution of all materials associated with this article)
  • Received:2022-01-09 Accepted:2022-03-02 Online:2022-06-01 Published:2022-06-15

Abstract:

Doubled haploid (DH) technology is used to obtain homozygous lines in a single generation, a technique that significantly accelerates the crop breeding trajectory. Traditionally, in vitro culture is used to generate DHs, but this technique is limited by species and genotype recalcitrance. In vivo haploid induction (HI) through seed is widely and efficiently used in maize and was recently extended to several other crops. Here we show that in vivo HI can be triggered by mutation of DMP maternal haploid inducer genes in allopolyploid (allotetraploid) Brassica napus and Nicotiana tabacum. We developed a pipeline for selection of DMP orthologs for clustered regularly interspaced palindromic repeats mutagenesis and demonstrated average amphihaploid induction rates of 2.4% and 1.2% in multiple B. napus and N. tabacum genotypes, respectively. These results further confirmed the HI ability of DMP gene in polyploid dicot crops. The DMP-HI system offers a novel DH technology to facilitate breeding in these crops. The success of this approach and the conservation of DMP genes in dicots suggest the broad applicability of this technique in other dicot crops.

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