J Integr Plant Biol ›› 2022, Vol. 64 ›› Issue (6): 1196-1211.DOI: 10.1111/jipb.13254

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  • 收稿日期:2022-01-10 接受日期:2022-03-17 出版日期:2022-06-01 发布日期:2022-06-15

Ascorbate peroxidase 1 confers resistance to southern corn leaf blight in maize

Jinghua Zhang1†, Xingmeng Jia1†, Guan‐Feng Wang2, Shijun Ma2, Shunxi Wang1, Qin Yang3, Xueyan Chen1, Yuqian Zhang1,4, Yajing Lyu1, Xiaoxu Wang1, Jiawei Shi1, Yangtao Zhao1, Yanhui Chen1 and Liuji Wu1*   

  1. 1 National Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China
    2 The Key Laboratory of Plant Development and Environmental Adaptation Biologym, Ministry of Education, School of Life Sciences, Shandong University, Qingdao 266237, China
    3 State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy, Northwest A&F University, Yangling 712100, China
    4 School of Environmental and Rural Science, University of New England, Armidale 2351, NSW, Australia

    † These authors contributed equally to this work.
    * Correspondence: Liuji Wu (wlj200120@163.com)
  • Received:2022-01-10 Accepted:2022-03-17 Online:2022-06-01 Published:2022-06-15

Abstract:

Southern corn leaf blight (SCLB), caused by Bipolaris maydis, is one of the most devastating diseases affecting maize production. However, only one SLCB resistance gene, conferring partial resistance, is currently known, underscoring the importance of isolating new SCLB resistance-related genes. Here, we performed a comparative proteomic analysis and identified 258 proteins showing differential abundance during the maize response to B. maydis. These proteins included an ascorbate peroxidase (Zea mays ascorbate peroxidase 1 (ZmAPX1)) encoded by a gene located within the mapping interval of a previously identified quantitative trait locus associated with SCLB resistance. ZmAPX1 overexpression resulted in lower H2O2 accumulation and enhanced resistance against B. maydis. Jasmonic acid (JA) contents and transcript levels for JA biosynthesis and responsive genes increased in ZmAPX1-overexpressing plants infected with B. maydis, whereas Zmapx1 mutants showed the opposite effects. We further determined that low levels of H2O2 are accompanied by an accumulation of JA that enhances SCLB resistance. These results demonstrate that ZmAPX1 positively regulates SCLB resistance by decreasing H2O2 accumulation and activating the JA-mediated defense signaling pathway. This study identified ZmAPX1 as a potentially useful gene for increasing SCLB resistance. Furthermore, the generated data may be relevant for clarifying the functions of plant APXs.

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