J Integr Plant Biol ›› 2022, Vol. 64 ›› Issue (7): 1430-1447.DOI: 10.1111/jipb.13271

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  • 收稿日期:2021-11-21 接受日期:2022-04-26 出版日期:2022-07-01 发布日期:2022-07-14

DEAP1 encodes a fasciclin-like arabinogalactan protein required for male fertility in rice

Dan Zhou1,2†, Ting Zou1,2†, Kaixuan Zhang2, Pingping Xiong2, Fuxing Zhou2, Hao Chen2, Gongwen Li2, Kaiyou Zheng2, Yuhao Han2, Kun Peng2, Xu Zhang2, Shangyu Yang2, Qiming Deng1,2, Shiquan Wang1,2, Jun Zhu1,2, Yueyang Liang1,2, Changhui Sun2, Xiumei Yu3, Huainian Liu1,2, Lingxia Wang1,2, Ping Li1,2* and Shuangcheng Li1,2*   

  1. 1 State Key Laboratory of Hybrid Rice, Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China
    2 State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China
    3 College of Resource, Sichuan Agricultural University, Chengdu 611130, China

    † These authors contributed equally to this work.
    * Correspondences: Shuangcheng Li (lisc926105@163.com; Dr. Li is fully responsible for the distributions of all materials associated with this article); Ping Li (liping6575@163.com)
  • Received:2021-11-21 Accepted:2022-04-26 Online:2022-07-01 Published:2022-07-14

Abstract:

Arabinogalactan proteins (AGPs) are widely distributed in plant cells. Fasciclin-like AGPs (FLAs) belong to a subclass of AGPs that play important roles in plant growth and development. However, little is known about the biological functions of rice FLA. Herein, we report the identification of a male-sterile mutant of DEFECTIVE EXINE AND APERTURE PATTERNING1 (DEAP1) in rice. The deap1 mutant anthers produced aberrant pollen grains with defective exine formation and a flattened aperture annulus and exhibited slightly delayed tapetum degradation. DEAP1 encodes a plasma membrane-associated member of group III plant FLAs and is specifically and temporally expressed in reproductive cells and the tapetum layer during male development. Gene expression studies revealed reduced transcript accumulation of genes related to exine formation, aperture patterning, and tapetum development in deap1 mutants. Moreover, DEAP1 may interact with two rice D6 PROTEIN KINASE-LIKE3s (OsD6PKL3s), homologs of a known Arabidopsis aperture protein, to affect rice pollen aperture development. Our findings suggested that DEAP1 is involved in male reproductive development and may affect exine formation and aperture patterning, thereby providing new insights into the molecular functions of plant FLAs in male fertility.

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