J Integr Plant Biol ›› 2022, Vol. 64 ›› Issue (11): 2135-2149.DOI: 10.1111/jipb.13343

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  • 收稿日期:2022-07-10 接受日期:2022-08-10 出版日期:2022-11-01 发布日期:2022-11-17

The plant circadian clock regulates autophagy rhythm through transcription factor LUX ARRHYTHMO

Ming‐Kang Yang1,2,3, Xiao‐Jie Zhu1, Chu‐Min Chen1, Xu Guo1, Shu‐Xuan Xu1, Ya‐Rou Xu1, Shen‐Xiu Du1, Shi Xiao4, Bernd Mueller‐Roeber5,6, Wei Huang1,2,3* and Liang Chen1,2,3*   

  1. 1 State Key Laboratory for Conservation and Utilization of Subtropical Agro‐Bioresources, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
    2 Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China
    3 Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, South China Agricultural University, Guangzhou 510642, China
    4 State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat‐Sen University, Guangzhou 510275, China
    5 Department of Molecular Biology, Institute of Biochemistry and Biology, University of Potsdam, 14476 Potsdam‐Golm, Germany
    6 Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam‐Golm, Germany

    *Correspondences: Liang Chen (chenliang@scau.edu.cn; Dr. Chen is fully responsible for the distribution of all materials associated with this article); Wei Huang (weihuang@scau.edu.cn)
  • Received:2022-07-10 Accepted:2022-08-10 Online:2022-11-01 Published:2022-11-17

Abstract:

Autophagy is an evolutionarily conserved degradation pathway in eukaryotes; it plays a critical role in nutritional stress tolerance. The circadian clock is an endogenous timekeeping system that generates biological rhythms to adapt to daily changes in the environment. Accumulating evidence indicates that the circadian clock and autophagy are intimately interwoven in animals. However, the role of the circadian clock in regulating autophagy has been poorly elucidated in plants. Here, we show that autophagy exhibits a robust circadian rhythm in both light/dark cycle (LD) and in constant light (LL) in Arabidopsis. However, autophagy rhythm showed a different pattern with a phase-advance shift and a lower amplitude in LL compared to LD. Moreover, mutation of the transcription factor LUX ARRHYTHMO (LUX) removed autophagy rhythm in LL and led to an enhanced amplitude in LD. LUX represses expression of the core autophagy genes ATG2, ATG8a, and ATG11 by directly binding to their promoters. Phenotypic analysis revealed that LUX is responsible for improved resistance of plants to carbon starvation, which is dependent on moderate autophagy activity. Comprehensive transcriptomic analysis revealed that the autophagy rhythm is ubiquitous in plants. Taken together, our findings demonstrate that the LUX-mediated circadian clock regulates plant autophagy rhythms.

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