J Integr Plant Biol ›› 2024, Vol. 66 ›› Issue (4): 787-809.DOI: 10.1111/jipb.13641

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  • 收稿日期:2024-01-29 接受日期:2024-02-26 出版日期:2024-04-01 发布日期:2024-04-13

The RING zinc finger protein LbRZF1 promotes salt gland development and salt tolerance in Limonium bicolor

Zongran Yang1†, Ziwei Zhang1†, Ziqi Qiao1†, Xueying Guo1, Yixuan Wen1, Yingxue Zhou1, Chunliang Yao1, Hai Fan1, Baoshan Wang1* and Guoliang Han1,2,3*   

  1. 1. Shandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Ji'nan 250014, China;
    2. National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Agricultural High-tech Industrial Demonstration Area of the Yellow River Delta of Shandong Province, Dongying 257000, China;
    3. Dongying Institute, Shandong Normal University, Dongying 257000, China
    †These authors contributed equally to this work.
    *Correspondences:Baoshan Wang(bswang@sdnu.edu.cn);Guoliang Han(gl_han@sdnu.edu.cn, Dr. Han is fully responsible for the distributions of all materials associated with this article)
  • Received:2024-01-29 Accepted:2024-02-26 Online:2024-04-01 Published:2024-04-13

Abstract: The recretohalophyte Limonium bicolor thrives in high-salinity environments because salt glands on the above-ground parts of the plant help to expel excess salt. Here, we characterize a nucleus-localized C3HC4 (RING-HC)-type zinc finger protein of L. bicolor named RING ZINC FINGER PROTEIN 1 (LbRZF1). LbRZF1 was expressed in salt glands and in response to NaCl treatment. LbRZF1 showed no E3 ubiquitin ligase activity. The phenotypes of overexpression and knockout lines for LbRZF1 indicated that LbRZF1 positively regulated salt gland development and salt tolerance in L. bicolor. lbrzf1 mutants had fewer salt glands and secreted less salt than did the wild-type, whereas LbRZF1-overexpressing lines had opposite phenotypes, in keeping with the overall salt tolerance of these plants. A yeast two-hybrid screen revealed that LbRZF1 interacted with LbCATALASE2 (LbCAT2) and the transcription factor LbMYB113, leading to their stabilization. Silencing of LbCAT2 or LbMYB113 decreased salt gland density and salt tolerance. The heterologous expression of LbRZF1 in Arabidopsis thaliana conferred salt tolerance to this non-halophyte. We also identified the transcription factor LbMYB48 as an upstream regulator of LbRZF1 transcription. The study of LbRZF1 in the regulation network of salt gland development also provides a good foundation for transforming crops and improving their salt resistance.

Key words: LbRZF1, Limonium bicolor, molecular mechanism, salt gland development, salt tolerance

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