J Integr Plant Biol ›› 2025, Vol. 67 ›› Issue (1): 117-134.DOI: 10.1111/jipb.13804  cstr: 32098.14.jipb.13804

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  • 收稿日期:2024-09-05 接受日期:2024-10-18 出版日期:2025-01-01 发布日期:2025-01-15

The PtobZIP55–PtoMYB170 module regulates the wood anatomical and chemical properties of Populus tomentosa in acclimation to low nitrogen availability

Jiangting Wu1†, Shurong Deng1†, Yang Wang1, Chenlin Jia1, Jia Wei1, Mengyan Zhou1, Dongyue Zhu1, Zhuorong Li1, Payam Fayyaz2, Zhi‐Bin Luo1,3,4*, Jing Zhou1* and Wenguang Shi1*   

  1. 1. State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
    2. Forest, Range and Watershed Management Department, Agriculture and Natural Resources Faculty, Yasouj University, Yasuj 75919 63179, Iran
    3. Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry, Beijing 100091, China
    4. Comprehensive Experimental Center of Chinese Academy of Forestry in Yellow River Delta, Dongying, Shandong 257000, China
    †These authors contributed equally to this work.
    *Correspondences: Wenguang Shi (swg0911@126.com); Jing Zhou (gaha2008@126.com); Zhi‐Bin Luo (luozbbill@163.com, Dr. Luo is fully responsible for the distribution of all materials associated with this article)
  • Received:2024-09-05 Accepted:2024-10-18 Online:2025-01-01 Published:2025-01-15
  • Supported by:
    This work was supported by the National Key Research and Development Program of China (Grant nos. 2022YFD2201400 and 2016YFD0600105), the National Natural Science Foundation of China (Grant nos. 32371826 and 32371841), and the Key Research and Development Program of Shandong Province (Grant no. 2023CXPT026).

Abstract: Poplar plantations are often established on nitrogen-poor land, and poplar growth and wood formation are constrained by low nitrogen (LN) availability. However, the molecular mechanisms by which specific genes regulate wood formation in acclimation to LN availability remain unclear. Here, we report a previously unrecognized module, basic region/leucine zipper 55 (PtobZIP55)–PtoMYB170, which regulates the wood formation of Populus tomentosa in acclimation to LN availability. PtobZIP55 was highly expressed in poplar wood and induced by LN. Altered wood anatomical properties and increased lignification were detected in PtobZIP55-overexpressing poplars, whereas the opposite results were detected in PtobZIP55-knockout poplars. Molecular and transgenic analyses revealed that PtobZIP55 directly binds to the promoter sequence of PtoMYB170 to activate its transcription. The phenotypes of PtoMYB170 transgenic poplars were similar to those of PtobZIP55 transgenic poplars under LN conditions. Further molecular analyses revealed that PtoMYB170 directly bound the promoter sequences of lignin biosynthetic genes to activate their transcription to increase lignin concentrations in LN-treated poplar wood. These results suggest that PtobZIP55 activates PtoMYB170 transcription, which in turn positively regulates lignin biosynthetic genes, increasing lignin deposition in the wood of P. tomentosa in the context of acclimation to LN availability.

Key words: lignin biosynthesis, nitrogen deficiency, Populus, secondary xylem, transcriptional regulation, wood formation

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