J Integr Plant Biol

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  • 收稿日期:2024-09-06 接受日期:2025-02-22

RNA interference-based dsRNA application confers prolonged protection against rice blast and viral diseases, offering a scalable solution for enhanced crop disease management

Pan Chen1†, Ying Lan2†, Shaochen Ding1, Ruonan Du1, Xiaoxiao Hu1, Han Zhang1, Hanxi Yu1, Le Xu1, Chenyang Li2, Feng lin2, Linlin Du2, Isashova Umida3, Rumiana Ray4, Tong Liu5, You Liang6, Dongdong Niu1, Hongxia Liu1, Tong Zhou2 and Hongwei Zhao1*   

  1. 1. State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China
    2. Key Laboratory of Food Quality and Safety, Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
    3. Department of plant protection, Andijan agricultural and Agrotechnology Institute, Kuyganyor 170600, Uzbekistan
    4. School of Biosciences, University of Nottingham, Sutton Bonington CampusNottingham NG7 2RD, UK
    5. Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, College of Plant Protection, Hainan University, Haikou 570228, China
    6. College of Agricultural, Yangzhou University, Yangzhou 225009, China

    †These authors contributed equally to this work.
    *Correspondence: Hongwei Zhao (hzhao@njau.edu.cn)
  • Received:2024-09-06 Accepted:2025-02-22
  • Supported by:
    This work was supported by a grant from a Natural Science Foundation of China program (32372556) and a Yangtze River Delta Science and Technology Innovation Consortium Key Research Project (24CSJ140200) to H.Z., as well as a Jiangsu Agricultural Science and Technology Innovation Fund (CX [22] 3019) to Y.L.

Abstract: Rice production is severely impacted by pathogens such as Magnaporthe oryzae and the rice stripe virus (RSV). Ineffectiveness in controlling viruses and the excessive use of fungicides have proven traditional chemical pesticides increasingly inadequate. RNA interference (RNAi) represents a cutting-edge approach for combating crop diseases, especially in rice. This study addresses the critical gap in scalable, effective RNAi-based rice disease management by exploring the potential of spray-applied small RNA (sRNA) and double-stranded RNA (dsRNA) molecules. We utilized dsRNAs produced by in vitro transcription and bacterial expression systems and employed layered double hydroxides (LDH) to enhance RNA stability, absorption, and efficacy. Our research demonstrated that modified sRNAs could effectively penetrate M. oryzae cell membranes and inhibit conidial germination and appressorium formation, while LDH-conjugated dsRNAs provided prolonged and enhanced protection against both rice blast and rice stripe diseases. Most importantly, dsRNA treatments resulted in improved agronomic traits or increased crop yields by protecting against blast and stripe diseases. This study also validated the compatibility of these RNA molecules with industrial production methods, highlighting their potential as a scalable and eco-friendly option for managing crop diseases at the gene level. This work not only offers a new direction for rice disease control but also provides a foundation for the broader application of RNAi technology in agricultural pest management.

Key words: gene silence, MoPth11, plant protection, rice blast, rice stripe disease, RNAi, RSV, SIGS, small RNA

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