J Integr Plant Biol ›› 2025, Vol. 67 ›› Issue (4): 1009-1027.DOI: 10.1111/jipb.13897

• • 上一篇    下一篇

  

  • 收稿日期:2024-06-20 接受日期:2025-02-28 出版日期:2025-04-01 发布日期:2025-04-28

FaNAC047-FaNAC058 module coordinately promotes chlorophyll degradation and reactive oxygen species production during heat-induced leaf senescence in tall fescue

Liwen Cao1, Yao Chen1,2, Kai Xiao1,2, Liang Chen1,2,3,*   

  1. 1. State Key Laboratory of Plant Diversity and Specialty Crops, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China;
    2. University of Chinese Academy of Science, Beijing 100049, China;
    3. Academician Workstation of Agricultural High-tech Industrial Area of the Yellow River Delta, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Dongying 257300, China
  • Received:2024-06-20 Accepted:2025-02-28 Online:2025-04-01 Published:2025-04-28
  • Contact: *Liang Chen (chenliang888@wbgcas.cn)
  • Supported by:
    National Natural Science Foundation of China (NSFC) (Grant Nos. 32471765, 32101430, and 32441039), the Major Science and Technology Innovation Project of Shandong Province (No. 2022LZGC018), the National Science Foundation for Distinguished Young Scholars of Hubei Province (No. 2021CFA060), International Science and Technology Cooperation Project of Hubei Province (No. 2021EHB021), and the Science & Technology Specific Projects in Agricultural Hightech Industrial Demonstration Area of the Yellow River Delta (No. 2022SZX13). We thank Professor Jianye Chen (South China Agricultural University) for providing pGreenII BD-62- SK and pGreenII BD-62-SK-VP16 vectors.

Abstract: Leaf senescence can be triggered by various abiotic stresses. Among these, heat stress emerges as a pivotal environmental factor, particularly in light of the predicted rise in global temperatures. However, the molecular mechanism underlying heat-induced leaf senescence remains largely unexplored. As a cool-season grass species, tall fescue (Festuca arundinacea) is an ideal and imperative material for investigating heat-induced leaf senescence because heat stress easily triggers leaf senescence to influence its forage yield and turf quality. Here, we investigated the role of FaNAC047 in heat-induced leaf senescence. Overexpression of FaNAC047 promoted heat-induced leaf senescence in transgenic tall fescue that was evidenced by a more seriously destructive photosystem and higher accumulation of reactive oxygen species (ROS), whereas knockdown of FaNAC047 delayed leaf senescence. Further protein-DNA interaction assays indicated that FaNAC047 directly activated the transcriptions of NON-YELLOW COLORING 1 (FaNYC1), NYC1-like (FaNOL), and STAY-GREEN (FaSGR) but directly inhibited Catalases 2 (FaCAT2) expression, thereby promoting chlorophyll degradation and ROS accumulation. Subsequently, protein-protein interaction assays revealed that FaNAC047 physically interacted with FaNAC058 to enhance its regulatory effect on FaNYC1, FaNOL, FaSGR, and FaCAT2. Additionally, FaNAC047 could transcriptionally activate FaNAC058 expression to form a regulatory cascade, driving senescence progression. Consistently, the knockdown of FaNAC058 significantly delayed heat-induced leaf senescence. Collectively, our results reveal that FaNAC047-FaNAC058 module coordinately mediates chlorophyll degradation and ROS production to positively regulate heat-induced leaf senescence. The findings illustrate the molecular network of heat-induced leaf senescence for breeding heat-resistant plants.

Key words: chlorophyll catabolic genes, FaCAT2, FaNAC047-FaNAC058 module, heat-induced leaf senescence, ROS production, tall fescue

[an error occurred while processing this directive]