Abstract:

A molecular genetic map of Brassica campestris L. (syn. B. rapa) was constructed based on the segregation of 99 RAPDs (random amplified polymorphic DNAs) markers from eighty-four 10-base random primers using DNA samples extracted from F2 population of turnip (B． campestris L. ssp. rapifera Metzg) × Chinese cabbage (B． campestris L. ssp. pekinensis Lour. Olsson). This genetic map covered 1 632.4 cM (centiMorgan) genome (Kosambi Function) with 16.5 cM mean intervals between flanking markers and defined thirteen linkage groups, in which the longest linkage group is 267.5 cM with 20.6 cM mean interval and the shortest linkage group is 62.2 cM with 15.6 cM mean interval. The size and distribution of linkage groups in this map is similar to other RFLP maps and karyotype data in B. campestris.

中国白菜RAPD分子遗传图谱的构建
张鲁刚¹ 王鸣¹  陈杭² 刘玲²

（1. 西北农林科技大学，陕西杨凌712100；2. 国家蔬菜工程技术中心，北京100080）

摘要：以芜菁 (Brassicacampestris L .ssp .rapifera Metzg)和结球白菜 (B .campestris L .ssp .pekinensis (Lour.)Olsson )杂交的F2 群体为试材 ,采用RAPD标记 ,用 84个 10核苷酸随机引物 ,构建了白菜的RAPD遗传图谱。该图谱覆盖基因组的 16 32 .4cM (centiMorgan) ,标记间的平均间隔为 16 .5cM。其中最长的连锁群为 2 6 7.5cM ,最短的连锁群为6 2 .2cM。该图谱的大小及连锁群的分布与细胞学资料比较一致。

关键词： RAPD；分子遗传图谱；白菜
 

Key words: RAPD, molecular linkage map, Brassica campestris