Abstract:

The effect of phospholipase C (PLC) signaling pathway on lily (Lilium davidii Duch.) pollen tube elongation was examined by means of microinjection. Pollen tube elongation was inhibited by microinjecting antibodies against animal PLCβ1-3 or inositol-1,4,5-triphosphate receptor （IP3R2, 3）, but was not affected by antibodies against animal PLCβ4 or IP3R1. Pollen tube elongation was also stimulated significantly by microinjecting IP3. The results suggest that PLC-IP3 signaling pathway might present in pollen system and be involved in pollen tube growth.

PLC-IP3信号途径参与花粉管伸长调控的显微注射实验
王昕 崔素娟 马力耕 孙大业*

（河北师范大学分子细胞生物学研究室，石家庄050016）

摘要：以百合 (Liliumdavidii Duch .)花粉为材料 ,通过显微注射肌醇磷脂信使系统中重要组成成分或其抗体 ,研究该信使系统对花粉管伸长的影响。发现显微注射动物来源磷脂酶C(PLC) β1-3 抗体显著抑制花粉管的伸长生长 ,而注射PLCβ4 抗体对花粉管伸长无影响 ;显微注射三磷酸肌醇 (IP3 ) ,可显著促进花粉管伸长生长 ;显微注射动物来源的IP3 R2 、IP3 R3 抗体可显著抑制花粉管伸长生长 ,而注射IP3 R1抗体对花粉管伸长无影响。上述结果表明肌醇磷脂信号系统可能参与花粉管伸长生长过程。

关键词： 磷脂酶C；三磷酸肌醇；三磷酸肌醇受体；花粉管伸长；显微注射

Key words: phospholipase C, inositol-1,4,5-triphosphate, inositol-1,4,5-triphosphate receptor, pollen tube elongation, microinjection